Overview

  • Product nameAnti-AKAP3 antibody
    See all AKAP3 primary antibodies
  • Description
    Goat polyclonal to AKAP3
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity

    Predicted to work with: Human
  • Immunogen

    Synthetic peptide:

    TPLQLLDWLMVNL

    , corresponding to C terminal amino acids 841-853 of Human AKAP3 .

  • Positive control
    • Human Testis lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline. pH 7.3
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab19046 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 95 kDa).
ELISA 1/32000.

Target

  • FunctionMay function as a regulator of both motility- and head-associated functions such as capacitation and the acrosome reaction.
  • Tissue specificityTestis specific; only expressed in spermatids.
  • Sequence similaritiesBelongs to the AKAP110 family.
  • DomainRII-binding site, predicted to form an amphipathic helix, could participate in protein-protein interactions with a complementary surface on the R-subunit dimer.
  • Post-translational
    modifications
    Phosphorylated on tyrosine residues.
  • Cellular localizationCytoplasmic vesicle > secretory vesicle > acrosome. Ribs of the fibrous sheath in the principal piece of the sperm tail. Dorsal margin of the acrosomal segment.
  • Information by UniProt
  • Database links
  • Alternative names
    • A kinase (PRKA) anchor protein 3 antibody
    • A-kinase anchor protein 110 kDa antibody
    • A-kinase anchor protein 3 antibody
    • AKAP 110 antibody
    • AKAP-3 antibody
    • AKAP110 antibody
    • Akap3 antibody
    • AKAP3_HUMAN antibody
    • Cancer/testis antigen 82 antibody
    • CT82 antibody
    • Fibrous sheath protein of 95 kDa antibody
    • Fibrousheathin 1 antibody
    • Fibrousheathin I antibody
    • Fibrousheathin-1 antibody
    • FSP95 antibody
    • PRKA3 antibody
    • protein kinase A binding protein AKAP 110 antibody
    • Protein kinase A-anchoring protein 3 antibody
    • SOB 1 antibody
    • SOB1 antibody
    • Sperm oocyte binding protein 1 antibody
    • Sperm oocyte-binding protein antibody
    see all

Anti-AKAP3 antibody images



  • Predicted band size : 95 kDa


    ab19046 (1µg/ml) staining of Human Testis lysate (35µg protein in RIPA buffer) approx. 90kDa. Primary incubation was 1 hour. Detected by chemiluminescence.

References for Anti-AKAP3 antibody (ab19046)

ab19046 has not yet been referenced specifically in any publications.

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Thank you for contacting us with your question.

We have not tested either of these antibodies in immunofluorescent staining yet, so we do not have an optimized dilution factor for them. I would recommend titrating the antibodies from 1-5 ug/m...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"