Overview

  • Product name
  • Description
    Goat polyclonal to AKAP3
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity

    Predicted to work with: Human
  • Immunogen

    Synthetic peptide:

    TPLQLLDWLMVNL

    , corresponding to C terminal amino acids 841-853 of Human AKAP3 .

  • Positive control
    • Human Testis lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline. pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab19046 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 95 kDa).
ELISA 1/32000.

Target

  • Function
    May function as a regulator of both motility- and head-associated functions such as capacitation and the acrosome reaction.
  • Tissue specificity
    Testis specific; only expressed in spermatids.
  • Sequence similarities
    Belongs to the AKAP110 family.
  • Domain
    RII-binding site, predicted to form an amphipathic helix, could participate in protein-protein interactions with a complementary surface on the R-subunit dimer.
  • Post-translational
    modifications
    Phosphorylated on tyrosine residues.
  • Cellular localization
    Cytoplasmic vesicle > secretory vesicle > acrosome. Ribs of the fibrous sheath in the principal piece of the sperm tail. Dorsal margin of the acrosomal segment.
  • Information by UniProt
  • Database links
  • Alternative names
    • A kinase (PRKA) anchor protein 3 antibody
    • A-kinase anchor protein 110 kDa antibody
    • A-kinase anchor protein 3 antibody
    • AKAP 110 antibody
    • AKAP-3 antibody
    • AKAP110 antibody
    • Akap3 antibody
    • AKAP3_HUMAN antibody
    • Cancer/testis antigen 82 antibody
    • CT82 antibody
    • Fibrous sheath protein of 95 kDa antibody
    • Fibrousheathin 1 antibody
    • Fibrousheathin I antibody
    • Fibrousheathin-1 antibody
    • FSP95 antibody
    • PRKA3 antibody
    • protein kinase A binding protein AKAP 110 antibody
    • Protein kinase A-anchoring protein 3 antibody
    • SOB 1 antibody
    • SOB1 antibody
    • Sperm oocyte binding protein 1 antibody
    • Sperm oocyte-binding protein antibody
    see all

Anti-AKAP3 antibody images



  • Predicted band size : 95 kDa


    ab19046 (1µg/ml) staining of Human Testis lysate (35µg protein in RIPA buffer) approx. 90kDa. Primary incubation was 1 hour. Detected by chemiluminescence.

References for Anti-AKAP3 antibody (ab19046)

ab19046 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us with your question.

We have not tested either of these antibodies in immunofluorescent staining yet, so we do not have an optimized dilution factor for them. I would recommend titrating the antibodies from 1-5 ug/m...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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