Overview

  • Product name
    Anti-AKAP3 antibody [EPR11261]
    See all AKAP3 primary antibodies
  • Description
    Rabbit Monoclonal [EPR11261] to AKAP3
  • Tested applications
    Suitable for: WB, ICC/IF, Flow Cytmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human AKAP3 .
    Database link: O75969

  • Positive control
    • Y79, HuT-78, HeLa, 293T, NIH 3T3, NCI-H460 and Jurkat cell lysates; HeLa cells; Permeabilized Jurkat cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 50% Glycerol, 0.05% BSA
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR11261
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab170856 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 95 kDa.
ICC/IF 1/250 - 1/500.
Flow Cyt 1/100 - 1/500. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function
      May function as a regulator of both motility- and head-associated functions such as capacitation and the acrosome reaction.
    • Tissue specificity
      Testis specific; only expressed in spermatids.
    • Sequence similarities
      Belongs to the AKAP110 family.
    • Domain
      RII-binding site, predicted to form an amphipathic helix, could participate in protein-protein interactions with a complementary surface on the R-subunit dimer.
    • Post-translational
      modifications
      Phosphorylated on tyrosine residues.
    • Cellular localization
      Cytoplasmic vesicle > secretory vesicle > acrosome. Ribs of the fibrous sheath in the principal piece of the sperm tail. Dorsal margin of the acrosomal segment.
    • Information by UniProt
    • Database links
    • Alternative names
      • A kinase (PRKA) anchor protein 3 antibody
      • A-kinase anchor protein 110 kDa antibody
      • A-kinase anchor protein 3 antibody
      • AKAP 110 antibody
      • AKAP-3 antibody
      • AKAP110 antibody
      • Akap3 antibody
      • AKAP3_HUMAN antibody
      • Cancer/testis antigen 82 antibody
      • CT82 antibody
      • Fibrous sheath protein of 95 kDa antibody
      • Fibrousheathin 1 antibody
      • Fibrousheathin I antibody
      • Fibrousheathin-1 antibody
      • FSP95 antibody
      • PRKA3 antibody
      • protein kinase A binding protein AKAP 110 antibody
      • Protein kinase A-anchoring protein 3 antibody
      • SOB 1 antibody
      • SOB1 antibody
      • Sperm oocyte binding protein 1 antibody
      • Sperm oocyte-binding protein antibody
      see all

    Images

    • Immunofluorescent analysis of HeLa cells labeling AKAP3 with ab170856 at 1/250 dilution (red). DAPI nuclear staining (blue).

    • Flow cytometric analysis of permeabilized Jurkat cells labeling AKAP3 with ab170856 at 1/100 dilution (red) or a rabbit IgG (negative) (green).

    • All lanes : Anti-AKAP3 antibody [EPR11261] (ab170856) at 1/1000 dilution

      Lane 1 : Y79 cell lysate
      Lane 2 : HuT-78 cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : 293T cell lysate
      Lane 5 : NIH 3T3 cell lysate
      Lane 6 : NCI-H460 cell lysate
      Lane 7 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 95 kDa

    References

    This product has been referenced in:
    • Xu Y  et al. Fluorescence- and magnetic-activated cell sorting strategies to separate spermatozoa involving plural contributors from biological mixtures for human identification. Sci Rep 6:36515 (2016). ICC/IF, WB ; Human . Read more (PubMed: 27857155) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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