Overview

  • Product nameAnti-AKAP4 antibody
  • Description
    Mouse monoclonal to AKAP4
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: MMAYSDTTMM SDDIDWLRSH RGVCKVDLYN PEGQQDQDRK VICFVDVSTL NVEDKDYKDA ASSSSEGNLN LGSLEEKEII VIKDTEKKDQ SKTEGSVCLF , corresponding to amino acids 1-101 of Human AKAP4

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • IsotypeIgG2a
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab56551 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 1 - 5 µg/ml.

Target

  • FunctionMajor structural component of sperm fibrous sheath. Plays a role in sperm motility.
  • Tissue specificityTestis specific; only expressed in round spermatids.
  • Sequence similaritiesBelongs to the AKAP110 family.
  • Developmental stagePost-meiotic phase of spermatogenesis.
  • DomainRI-alpha binding site, predicted to form an amphipathic helix, could participate in protein-protein interactions with a complementary surface on the R-subunit dimer.
  • Cellular localizationCell projection > cilium > flagellum. Localizes to the principle piece of the sperm flagellum.
  • Information by UniProt
  • Database links
  • Alternative names
    • A kinase (PRKA) anchor protein 4 antibody
    • A kinase anchor protein 4 antibody
    • A kinase anchor protein 82 kDa antibody
    • A-kinase anchor protein 4 antibody
    • A-kinase anchor protein 82 kDa antibody
    • AKAP 4 antibody
    • AKAP 82 antibody
    • AKAP-4 antibody
    • AKAP4 antibody
    • AKAP4_HUMAN antibody
    • AKAP82 antibody
    • FSC 1 antibody
    • FSC1 antibody
    • hAKAP 82 antibody
    • hAKAP82 antibody
    • HI antibody
    • Major sperm fibrous sheath protein antibody
    • p82 antibody
    • PRKA 4 antibody
    • PRKA4 antibody
    • Protein kinase A anchoring protein 4 antibody
    • Protein kinase A-anchoring protein 4 antibody
    • Testis specific gene HI antibody
    see all

Anti-AKAP4 antibody images

  • Western blot against tagged recombinant protein immunogen using ab56551 AKAP4 antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa
  • IHC image of ab56551 staining in human normal testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab56551, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-AKAP4 antibody (ab56551)

ab56551 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us with your question.

We have not tested either of these antibodies in immunofluorescent staining yet, so we do not have an optimized dilution factor for them. I would recommend titrating the antibodies from 1-5 ug/m...

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Thank you for your enquiry.

As far as we are aware, I am sorry to confirm that ab56551 has not been tested in endogenous samples in western blot, or on sperm samples.We are therefore not able to provide any further images and data on this oc...

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Thank you for your enquiry and for your patience .

With regard to AKAP4 monoclonal antibody, I can confirm that we have tested it in Western blot application. It could recognize the denaturalized form of recombinant tagged AKAP4 protein. We h...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"