Overview

  • Product name
  • Description
    Mouse monoclonal to AKAP4
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: MMAYSDTTMM SDDIDWLRSH RGVCKVDLYN PEGQQDQDRK VICFVDVSTL NVEDKDYKDA ASSSSEGNLN LGSLEEKEII VIKDTEKKDQ SKTEGSVCLF , corresponding to amino acids 1-101 of Human AKAP4

  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by July 2018 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. 

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Isotype
    IgG2a
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab56551 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 1 - 5 µg/ml.

Target

  • Function
    Major structural component of sperm fibrous sheath. Plays a role in sperm motility.
  • Tissue specificity
    Testis specific; only expressed in round spermatids.
  • Sequence similarities
    Belongs to the AKAP110 family.
  • Developmental stage
    Post-meiotic phase of spermatogenesis.
  • Domain
    RI-alpha binding site, predicted to form an amphipathic helix, could participate in protein-protein interactions with a complementary surface on the R-subunit dimer.
  • Cellular localization
    Cell projection > cilium > flagellum. Localizes to the principle piece of the sperm flagellum.
  • Information by UniProt
  • Database links
  • Alternative names
    • A kinase (PRKA) anchor protein 4 antibody
    • A kinase anchor protein 4 antibody
    • A kinase anchor protein 82 kDa antibody
    • A-kinase anchor protein 4 antibody
    • A-kinase anchor protein 82 kDa antibody
    • AKAP 4 antibody
    • AKAP 82 antibody
    • AKAP-4 antibody
    • AKAP4 antibody
    • AKAP4_HUMAN antibody
    • AKAP82 antibody
    • FSC 1 antibody
    • FSC1 antibody
    • hAKAP 82 antibody
    • hAKAP82 antibody
    • HI antibody
    • Major sperm fibrous sheath protein antibody
    • p82 antibody
    • PRKA 4 antibody
    • PRKA4 antibody
    • Protein kinase A anchoring protein 4 antibody
    • Protein kinase A-anchoring protein 4 antibody
    • Testis specific gene HI antibody
    see all

Anti-AKAP4 antibody images

  • Western blot against tagged recombinant protein immunogen using ab56551 AKAP4 antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa
  • IHC image of ab56551 staining in human normal testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab56551, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-AKAP4 antibody (ab56551)

ab56551 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us with your question.

We have not tested either of these antibodies in immunofluorescent staining yet, so we do not have an optimized dilution factor for them. I would recommend titrating the antibodies from 1-5 ug/m...

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Thank you for your enquiry.

As far as we are aware, I am sorry to confirm that ab56551 has not been tested in endogenous samples in western blot, or on sperm samples.We are therefore not able to provide any further images and data on this oc...

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Thank you for your enquiry and for your patience .

With regard to AKAP4 monoclonal antibody, I can confirm that we have tested it in Western blot application. It could recognize the denaturalized form of recombinant tagged AKAP4 protein. We h...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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