Overview

  • Product nameAnti-AKAP7 antibody
    See all AKAP7 primary antibodies
  • Description
    Rabbit polyclonal to AKAP7
  • SpecificityDetects gamma isoform only.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human, African Green Monkey
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Synthetic peptide, found within amino acids 127-176 of Human AKAP7

  • Positive control
    • Fetal Brain Lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab30987 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 37 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA 1/1.5625e+006.

Target

  • FunctionTargets the cAMP-dependent protein kinase (PKA) to the plasma membrane, and permits functional coupling to the L-type calcium channel.
  • Tissue specificityExpressed in brain, heart, lung, pancreas and skeletal muscle.
  • Cellular localizationApical cell membrane. Targeted predominantly to the apical membrane and Lateral cell membrane. Targeted predominantly to the lateral membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • A kinase (PRKA) anchor protein 7 antibody
    • A kinase anchor protein 18 kD antibody
    • A kinase anchor protein 7 antibody
    • A kinase anchor protein 7 isoform alpha antibody
    • A kinase anchor protein 7 isoform beta antibody
    • A kinase anchor protein 7 isoform gamma antibody
    • A kinase anchor protein 7 isoforms alpha and beta antibody
    • A kinase anchor protein 9 kDa antibody
    • A kinase anchoring protein 18 antibody
    • A-kinase anchor protein 18 kDa antibody
    • A-kinase anchor protein 7 isoforms alpha and beta antibody
    • AKA7A_HUMAN antibody
    • AKAP 18 antibody
    • AKAP 7 antibody
    • AKAP-7 isoforms alpha and beta antibody
    • AKAP15 antibody
    • AKAP18 antibody
    • Akap7 antibody
    • HGNC377 antibody
    • OTTHUMP00000040266 antibody
    • Protein kinase A anchoring protein 7 antibody
    • Protein kinase A anchoring protein 7 isoform alpha antibody
    • Protein kinase A anchoring protein 7 isoform beta antibody
    • Protein kinase A anchoring protein 7 isoform gamma antibody
    • Protein kinase A anchoring protein 7 isoforms alpha/beta antibody
    • Protein kinase A-anchoring protein 7 isoforms alpha/beta antibody
    see all

Anti-AKAP7 antibody images

  • Anti-AKAP7 antibody (ab30987) at 1.25 µg/ml (in 5% skim milk / PBS buffer) + fetal brain lysate

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 37 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)

References for Anti-AKAP7 antibody (ab30987)

ab30987 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample African Green Monkey Cell lysate - whole cell (COS-7 cells)
Specification COS-7 cells
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

B Blackman

Verified customer

Submitted Sep 26 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"