Overview

  • Product name
  • Description
    Goat polyclonal to AKAP8
  • Host species
    Goat
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Dog
  • Immunogen

    Synthetic peptide:

    DQGYGGYGAWSAG

    , corresponding to N terminal amino acids 2 - 14 of Human AKAP8.

  • Positive control
    • Human Liver lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline. pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab23930 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notes
    Peptide ELISA: 1/32000.
    WB: Use at a concentration of 1 - 3 µg/ml. Predicted molecular weight: 96 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Anchoring protein that mediates the subcellular compartmentation of cAMP-dependent protein kinase (PKA type II).
    • Tissue specificity
      Highly expressed in heart, liver, skeletal muscle, kidney and pancreas.
    • Sequence similarities
      Belongs to the AKAP95 family.
      Contains 2 C2H2 AKAP95-type zinc fingers.
    • Cellular localization
      Nucleus matrix. Associated with the nuclear matrix. Redistributed and detached from condensed chromatin during mitosis.
    • Information by UniProt
    • Database links
    • Alternative names
      • A kinase (PRKA) anchor protein 8 antibody
      • A kinase anchor protein 8 antibody
      • A kinase anchor protein 95 antibody
      • A kinase anchor protein 95 kDa antibody
      • A kinase anchor protein 95kDa antibody
      • A-kinase anchor protein 8 antibody
      • A-kinase anchor protein 95 kDa antibody
      • AKAP 8 antibody
      • AKAP 95 antibody
      • AKAP-8 antibody
      • AKAP-95 antibody
      • Akap8 antibody
      • AKAP8_HUMAN antibody
      • AKAP95 antibody
      • DKFZp586B1222 antibody
      see all

    Images

    • Anti-AKAP8 antibody (ab23930) at 1 µg/ml + Human Liver lysate (RIPA buffer, 35µg total protein per lane).

      Predicted band size: 96 kDa
      Observed band size: 100 kDa (why is the actual band size different from the predicted?)



      Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

    References

    This product has been referenced in:

    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab23930.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up