• Product nameAnti-AKAP8 antibody
    See all AKAP8 primary antibodies
  • Description
    Rabbit polyclonal to AKAP8
  • Tested applicationsSuitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Cow, Chimpanzee, Gorilla
  • Immunogen

    Synthetic peptide, corresponding to C terminal amino acids 642-692 of Human AKAP8 (NP_005849.1)

  • Positive control
    • HeLa and 293T whole cell lysates


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab72196 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 86 kDa (predicted molecular weight: 76 kDa).
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


  • FunctionAnchoring protein that mediates the subcellular compartmentation of cAMP-dependent protein kinase (PKA type II).
  • Tissue specificityHighly expressed in heart, liver, skeletal muscle, kidney and pancreas.
  • Sequence similaritiesBelongs to the AKAP95 family.
    Contains 2 C2H2 AKAP95-type zinc fingers.
  • Cellular localizationNucleus matrix. Associated with the nuclear matrix. Redistributed and detached from condensed chromatin during mitosis.
  • Information by UniProt
  • Database links
  • Alternative names
    • A kinase (PRKA) anchor protein 8 antibody
    • A kinase anchor protein 8 antibody
    • A kinase anchor protein 95 antibody
    • A kinase anchor protein 95 kDa antibody
    • A kinase anchor protein 95kDa antibody
    • A-kinase anchor protein 8 antibody
    • A-kinase anchor protein 95 kDa antibody
    • AKAP 8 antibody
    • AKAP 95 antibody
    • AKAP-8 antibody
    • AKAP-95 antibody
    • Akap8 antibody
    • AKAP8_HUMAN antibody
    • AKAP95 antibody
    • DKFZp586B1222 antibody
    see all

Anti-AKAP8 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma (left) and mouse teratoma (right) tissues labelling AKAP8 with ab72196 at 1/1000 (0.2µg/ml) and 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-AKAP8 antibody (ab72196) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 76 kDa

    Exposure time : 3 minutes
  • Immunoprecipitation of HeLa whole cell lysate (1 mg) using ab72196 at 3 µg/mg lysate. 20% of the immunoprecipitate was used for Western blot and bands were detected using ab72196 at 1 µg/ml. Bands were developed using chemiluminescence with an exposure time of 3 seconds.
    Lane 2 represents a Control IgG.

References for Anti-AKAP8 antibody (ab72196)

ab72196 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab72196.
Please use the links above to contact us or submit feedback about this product.