The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Peptide ELISA: 1/312500.
Use a concentration of 1.25 µg/ml. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use a concentration of 4 - 8 µg/ml.
AKAP8L could play a role in constitutive transport element (CTE)-mediated gene expression. It does not seem to be implicated in the binding of regulatory subunit II of PKA. It may be involved in nuclear envelope breakdown and chromatin condensation. It may regulate the initiation phase of DNA replication when associated with TMPO-beta.
Nuclear matrix. Cytoplasm. Note=Nuclear at steady state but shuttles between the nucleus and cytoplasm. Associated with the nuclear matrix.