Overview

  • Product nameAnti-AKAP8L antibody
    See all AKAP8L primary antibodies
  • Description
    Rabbit polyclonal to AKAP8L
  • Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Cow, Dog, Pig
  • Immunogen

    A region within synthetic peptide: GAVSPPPPPP PEEEEEGAVP LLGGALQRQI RGIPGLDVED DEEGGGGAP, corresponding to C terminal amino acids 598-646 of Human AKAP8L

  • Positive control
    • Jurkat cell lysate, human lung and human kidney tissue.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab51342 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

Peptide ELISA: 1/312500.

WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

Target

  • RelevanceAKAP8L could play a role in constitutive transport element (CTE)-mediated gene expression. It does not seem to be implicated in the binding of regulatory subunit II of PKA. It may be involved in nuclear envelope breakdown and chromatin condensation. It may regulate the initiation phase of DNA replication when associated with TMPO-beta.
  • Cellular localizationNuclear matrix. Cytoplasm. Note=Nuclear at steady state but shuttles between the nucleus and cytoplasm. Associated with the nuclear matrix.
  • Database links
  • Alternative names
    • A kinase (PRKA) anchor protein 8 like antibody
    • A kinase anchor protein 8 like antibody
    • AKAP 8L antibody
    • AKAP8 like protein antibody
    • DKFZp434L0650 antibody
    • HA95 antibody
    • HAP95 antibody
    • Helicase A binding protein 95 antibody
    • Homologous to AKAP95 protein antibody
    • NAKAP antibody
    • NAKAP95 antibody
    • Neighbor of A kinase anchoring protein 95 antibody
    • Neighbor of AKAP95 antibody
    see all

Anti-AKAP8L antibody images

  • Anti-AKAP8L antibody (ab51342) at 1.25 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG (1:100,000)

    Predicted band size : 72 kDa
    Observed band size : 72 kDa
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue labelling AKAP8L with ab51342 at 4-8µg/ml. Arrows indicate positively labelled alveolar cells. Magnification: 400X.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling AKAP8L with abAKAP8L at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of renal tubule. Magnification: 400X.

References for Anti-AKAP8L antibody (ab51342)

This product has been referenced in:

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"