Overview

  • Product nameAnti-AKAP9 antibody [17G10]
    See all AKAP9 primary antibodies
  • Description
    Mouse monoclonal [17G10] to AKAP9
  • Tested applicationsSuitable for: WB, ICC/IF, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Dog, Chimpanzee
  • Immunogen

    Synthetic peptide:

    QFRQRKAQSDGQSPS

    , corresponding to amino acids 31-45 of Human AKAP9

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, PBS
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • Clone number17G10
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab32679 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 µg/ml. Predicted molecular weight: 454 kDa.
ICC/IF Use a concentration of 4 µg/ml.
IP Use at an assay dependent concentration.
IHC-P 1/10 - 1/100.

Target

  • FunctionBinds to type II regulatory subunits of protein kinase A. Scaffolding protein that assembles several protein kinases and phosphatases on the centrosome and Golgi apparatus. May be required to maintain the integrity of the Golgi apparatus. Isoform 4 is associated with the N-methyl-D-aspartate receptor and is specifically found in the neuromuscular junction (NMJ) as well as in neuronal synapses, suggesting a role in the organization of postsynaptic specializations.
  • Tissue specificityWidely expressed. Isoform 4 is highly expressed in skeletal muscle and in pancreas.
  • Involvement in diseaseDefects in AKAP9 are the cause of long QT syndrome type 11 (LQT11) [MIM:611820]. Long QT syndromes are heart disorders characterized by a prolonged QT interval on the ECG and polymorphic ventricular arrhythmias. They cause syncope and sudden death in response to excercise or emotional stress. They can present with a sentinel event of sudden cardiac death in infancy.
  • DomainRII-binding site, predicted to form an amphipathic helix, could participate in protein-protein interactions with a complementary surface on the R-subunit dimer.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationCytoplasm. Cytoplasm > cytoskeleton > centrosome. Golgi apparatus. Cytoplasmic in parietal cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • A kinase (PRKA) anchor protein (yotiao) 9 antibody
    • A kinase (PRKA) anchor protein 9 antibody
    • A kinase anchor protein 350kDa antibody
    • A kinase anchor protein 450 kDa antibody
    • A kinase anchor protein 9 antibody
    • A kinase anchoring protein 350 antibody
    • A kinase anchoring protein 450 antibody
    • A-kinase anchor protein 350 kDa antibody
    • A-kinase anchor protein 450 kDa antibody
    • A-kinase anchor protein 9 antibody
    • AKAP 120-like protein antibody
    • AKAP 350 antibody
    • AKAP 450 antibody
    • AKAP 9 antibody
    • AKAP-9 antibody
    • AKAP120 like protein antibody
    • AKAP350 antibody
    • AKAP450 antibody
    • AKAP9 antibody
    • AKAP9_HUMAN antibody
    • Centrosome and Golgi localized PKN associated protein antibody
    • Centrosome and golgi localized protein antibody
    • Centrosome- and Golgi-localized PKN-associated protein antibody
    • CG NAP antibody
    • CG-NAP antibody
    • hgAKAP 350 antibody
    • hgAKAP350 antibody
    • Hyperion antibody
    • KIAA0803 antibody
    • Kinase N associated protein antibody
    • MU RMS 40.16A antibody
    • PRKA 9 antibody
    • PRKA9 antibody
    • Protein hyperion antibody
    • Protein kinase A anchoring protein 9 antibody
    • Protein kinase A-anchoring protein 9 antibody
    • Protein yotiao antibody
    • Yotiao antibody
    see all

Anti-AKAP9 antibody [17G10] images

  • Immunofluorescent analysis of AKAP9 in HeLa cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a AKAP9 monoclonal antibody (ab32679) at a dilution of 1:20 overnight at 4 C and incubated with a DyLight-488 conjugated secondary antibody. AKAP9 staining (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

  • Immunofluorescent analysis of AKAP9 in A431 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a AKAP9 monoclonal antibody (ab32679) at a dilution of 1:100 overnight at 4 C and incubated with a DyLight-488 conjugated secondary antibody. AKAP9 staining (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

  • Immunofluorescent analysis of AKAP9 in NIH-3T3 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a AKAP9 monoclonal antibody (ab32679) at a dilution of 1:100 overnight at 4 C and incubated with a DyLight-488 conjugated secondary antibody. AKAP9 staining (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

  • Immunohistochemistry was performed on normal biopsies of deparaffinized Human pancreas tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer and microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature and probed with a AKAP9 monoclonal antibody (ab32679) at a dilution of 1:20 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

References for Anti-AKAP9 antibody [17G10] (ab32679)

ab32679 has not yet been referenced specifically in any publications.

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