SpecificityThis anitbody reacts with the AKAP9 protein. On Western blots of fetal mouse brain, a clear single band at ~230kD is observed. On Western blots of AsPC-1 lysates and rat brain homogenates, multiple bands at other molecular weights have also been observed. Reactivity has been confirmed with AsPC-1 (human pancreatic adenocarcinoma) cell lysates and rat brain tissue homogenates, in addition to fetal mouse brain homogenates.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.1 - 1 µg/ml.
Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 454 kDa.
FunctionBinds to type II regulatory subunits of protein kinase A. Scaffolding protein that assembles several protein kinases and phosphatases on the centrosome and Golgi apparatus. May be required to maintain the integrity of the Golgi apparatus. Isoform 4 is associated with the N-methyl-D-aspartate receptor and is specifically found in the neuromuscular junction (NMJ) as well as in neuronal synapses, suggesting a role in the organization of postsynaptic specializations.
Tissue specificityWidely expressed. Isoform 4 is highly expressed in skeletal muscle and in pancreas.
Involvement in diseaseDefects in AKAP9 are the cause of long QT syndrome type 11 (LQT11) [MIM:611820]. Long QT syndromes are heart disorders characterized by a prolonged QT interval on the ECG and polymorphic ventricular arrhythmias. They cause syncope and sudden death in response to excercise or emotional stress. They can present with a sentinel event of sudden cardiac death in infancy.
DomainRII-binding site, predicted to form an amphipathic helix, could participate in protein-protein interactions with a complementary surface on the R-subunit dimer.
Post-translational modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.