Overview

  • Product name
  • Description
    Goat polyclonal to AKAP9
  • Specificity
    This antibody is expected to recognise three of the four reported isoforms (NP_005742; NP_671695; NP_671714) and MAY also recognise NP_671700 whose last 4 aa differ from the others.
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide:

    (C)SGSTTQFHAGMR

    , corresponding to C terminal amino acids 3899-3910 of Human AKAP9

  • Positive control
    • HepG2 cell line.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline. pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab31307 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notes
    Peptide ELISA: 1/32000.
    WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 450 kDa (predicted molecular weight: 454 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Binds to type II regulatory subunits of protein kinase A. Scaffolding protein that assembles several protein kinases and phosphatases on the centrosome and Golgi apparatus. May be required to maintain the integrity of the Golgi apparatus. Isoform 4 is associated with the N-methyl-D-aspartate receptor and is specifically found in the neuromuscular junction (NMJ) as well as in neuronal synapses, suggesting a role in the organization of postsynaptic specializations.
    • Tissue specificity
      Widely expressed. Isoform 4 is highly expressed in skeletal muscle and in pancreas.
    • Involvement in disease
      Defects in AKAP9 are the cause of long QT syndrome type 11 (LQT11) [MIM:611820]. Long QT syndromes are heart disorders characterized by a prolonged QT interval on the ECG and polymorphic ventricular arrhythmias. They cause syncope and sudden death in response to excercise or emotional stress. They can present with a sentinel event of sudden cardiac death in infancy.
    • Domain
      RII-binding site, predicted to form an amphipathic helix, could participate in protein-protein interactions with a complementary surface on the R-subunit dimer.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization
      Cytoplasm. Cytoplasm > cytoskeleton > centrosome. Golgi apparatus. Cytoplasmic in parietal cells.
    • Information by UniProt
    • Database links
    • Alternative names
      • A kinase (PRKA) anchor protein (yotiao) 9 antibody
      • A kinase (PRKA) anchor protein 9 antibody
      • A kinase anchor protein 350kDa antibody
      • A kinase anchor protein 450 kDa antibody
      • A kinase anchor protein 9 antibody
      • A kinase anchoring protein 350 antibody
      • A kinase anchoring protein 450 antibody
      • A-kinase anchor protein 350 kDa antibody
      • A-kinase anchor protein 450 kDa antibody
      • A-kinase anchor protein 9 antibody
      • AKAP 120-like protein antibody
      • AKAP 350 antibody
      • AKAP 450 antibody
      • AKAP 9 antibody
      • AKAP-9 antibody
      • AKAP120 like protein antibody
      • AKAP350 antibody
      • AKAP450 antibody
      • AKAP9 antibody
      • AKAP9_HUMAN antibody
      • Centrosome and Golgi localized PKN associated protein antibody
      • Centrosome and golgi localized protein antibody
      • Centrosome- and Golgi-localized PKN-associated protein antibody
      • CG NAP antibody
      • CG-NAP antibody
      • hgAKAP 350 antibody
      • hgAKAP350 antibody
      • Hyperion antibody
      • KIAA0803 antibody
      • Kinase N associated protein antibody
      • MU RMS 40.16A antibody
      • PRKA 9 antibody
      • PRKA9 antibody
      • Protein hyperion antibody
      • Protein kinase A anchoring protein 9 antibody
      • Protein kinase A-anchoring protein 9 antibody
      • Protein yotiao antibody
      • Yotiao antibody
      see all

    References for Anti-AKAP9 antibody (ab31307)

    ab31307 has not yet been referenced specifically in any publications.

    Product Wall

    Abreviews
    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Cell lysate - nuclear (CH12 mouse B cell line)
    Loading amount
    50 µg
    Specification
    CH12 mouse B cell line
    Treatment
    IL4, CD40, TGFbeta for 48 hours
    Gel Running Conditions
    Reduced Denaturing (6% SDS-PAGE)
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Jan 06 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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