AKIP overexpression 293T lysate (whole cell) (ab94297)

Overview

  • Product name
    AKIP overexpression 293T lysate (whole cell)
  • Description
    AKIP overexpression lysate
  • General notes
    ab94297 is a 293T cell transfected lysate in which Human AKIP has been transiently over-expressed using a pCMV-AKIP plasmid. The lysate is provided in 1 x Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • Tested applications
    Suitable for: WBmore details

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 6.80
    Constituent: 100% 1x Sample Buffer
  • Concentration information loading...
  • Purity
    Whole Cell Lysate
  • Research areas
  • Background
    Function: May act as a negative regulator of Aurora-A kinase, by down-regulation through proteasome-dependent degradation. Tissue specificity: Ubiquitously expressed and especially highly expressed in heart, skeletal muscle and testis.

Applications

Our Abpromise guarantee covers the use of ab94297 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.

Images

  • ab94297 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-AKIP antibody (ab67343) at 1/500 dilution

    Lane 1 : AKIP overexpression 293T lysate (whole cell) (ab94297)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

References

ab94297 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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