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Rabbit polyclonal to AKR1B1
HumanPredicted to work with:
Rat, Rabbit, Cow
Recombinant fragment, corresponding to a region within amino acids 1-316 of Human AKR1B1 (Uniprot ID: P15121).
- 293T, A431, HeLa, HepG2, MOLT4 and Raji whole cell lysates; HeLa cells; Human colon carcinoma tissue.
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 0.75% Glycine, 1.21% Tris, 10% Glycerol
Concentration information loading...
Immunogen affinity purified
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/500 - 1/3000. Predicted molecular weight: 36 kDa.
||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
||1/100 - 1/1000.
Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols with a broad range of catalytic efficiencies.
Highly expressed in embryonic epithelial cells (EUE) in response to osmotic stress.
Belongs to the aldo/keto reductase family.
Information by UniProt
- ADR antibody
- AKR1B 1 antibody
- Akr1b1 antibody
Western blot - Anti-AKR1B1 antibody (ab153897)
All lanes : Anti-AKR1B1 antibody (ab153897) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 36 kDa
10% SDS PAGE
Immunocytochemistry/ Immunofluorescence - Anti-AKR1B1 antibody (ab153897)
Immunofluorescent analysis of methanol-fixed HeLa cells labeling AKR1B1 with ab153897 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKR1B1 antibody (ab153897)
Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling AKR1B1 with ab153897 at 1/500 dilution.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"