Overview

  • Product nameAnti-AKR1B1 antibodySee all AKR1B1 primary antibodies ...
  • Description
    Rabbit polyclonal to AKR1B1
  • Tested applicationsWB, IHC-P, ICC/IF more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus of Human AKR1B1.

    (Peptide available as ab92665.)

  • Positive control
    • This antibody gave a positive signal in human kidney and placenta tissue lysates as well as the following whole cell lysates: HeLa; Jurkat; HEK293; Caco2; MEL-1. This antibody gave a positive result in IF/ICC when used in the following formaldehyde fixed cell lines: HepG2.

Properties

Applications

Our Abpromise guarantee covers the use of ab62795 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).Can be blocked with Human AKR1B1 peptide (ab92665).
IHC-P Use at an assay dependent concentration. PubMed: 23146748
ICC/IF Use a concentration of 5 µg/ml.

Target

  • FunctionCatalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols with a broad range of catalytic efficiencies.
  • Tissue specificityHighly expressed in embryonic epithelial cells (EUE) in response to osmotic stress.
  • Sequence similaritiesBelongs to the aldo/keto reductase family.
  • Cellular localizationCytoplasm.
  • Target information above from: UniProt accession P15121 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • ADR antibody
    • AKR1B 1 antibody
    • Akr1b1 antibody
    • Aldehyde reductase 1 antibody
    • Aldehyde reductase antibody
    • Aldo keto reductase family 1, member B1 antibody
    • Aldo-keto reductase family 1 member B1 antibody
    • aldo-keto reductase family 1, member B1 (aldose reductase) antibody
    • Aldose reductase antibody
    • aldr 1 antibody
    • ALDR_HUMAN antibody
    • ALDR1 antibody
    • aldr1 antibody
    • ALR2 antibody
    • AR antibody
    • Lii5 2 CTCL tumor antigen antibody
    • Low Km aldose reductase antibody
    • MGC1804 antibody
    see all

Anti-AKR1B1 antibody images

  • All lanes : Anti-AKR1B1 antibody (ab62795) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 4 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
    Lane 5 : Kidney (Human) Tissue Lysate - adult normal tissue (ab30203)
    Lane 6 : Placenta (Human) Tissue Lysate - adult normal tissue (ab29745)
    Lane 7 : MEL-1 (Human embryonic stem cell, male cell line) Whole Cell Lysate (ab27198)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 36 kDa
    Observed band size : 36 kDa
    Additional bands at : 44 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes
  • ab62795 staining AKR1B1 in Human endometrial cancer tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5% serum for 60 minutes at 25°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/100 in 5% Gaot serum in TPBS) for 20 hours at 4°C. A Goat anti-rabbit IgG+IgM H+L polyclonal (1/100) was used as the secondary antibody.

    See Abreview

  • ICC/IF image of ab62795 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab62795 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-AKR1B1 antibody (ab62795)

This product has been referenced in:
  • Hevir N  et al. Decreased levels of AKR1B1 and AKR1B10 in cancerous endometrium compared to adjacent non-cancerous tissue. Chem Biol Interact : (2012). WB, IHC-P ; Human . Read more (PubMed: 23146748) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (endometrial cancer)
Specification endometrial cancer
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: sodium citrate buffer (pH=6) in microwave oven
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

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Verified customer

Submitted Feb 13 2013

Application Western blot
Sample Human Tissue lysate - whole (endometrial cancer and adjacent control endometriu)
Loading amount 30 µg
Specification endometrial cancer and adjacent control endometriu
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Feb 12 2013

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