Overview

  • Product nameAnti-AKR1B10 antibody
    See all AKR1B10 primary antibodies
  • Description
    Rabbit polyclonal to AKR1B10
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-286 of Human AKR1B10 (NP_064695).

  • Positive control
    • MOLT4 and Raji whole cell lysates; HeLa cells; OVCAR3 xenograft; 293T, A431, H1299 and HepG2 cell lines

Properties

Applications

Our Abpromise guarantee covers the use of ab96417 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 36 kDa.
IHC-P 1/100 - 1/500.
ICC/IF 1/100 - 1/200.

Target

  • FunctionActs as all-trans-retinaldehyde reductase. Can efficiently reduce aliphatic and aromatic aldehydes, and is less active on hexoses (in vitro). May be responsible for detoxification of reactive aldehydes in the digested food before the nutrients are passed on to other organs.
  • Tissue specificityFound in many tissues. Highly expressed in small intestine, colon and adrenal gland.
  • Sequence similaritiesBelongs to the aldo/keto reductase family.
  • Cellular localizationLysosome. Secreted. Secreted through a lysosome-mediated non-classical pathway.
  • Information by UniProt
  • Database links
  • Alternative names
    • AK1BA_HUMAN antibody
    • AKR1B10 antibody
    • AKR1B11 antibody
    • AKR1B12 antibody
    • Aldo keto reductase family 1 member B10 antibody
    • aldo keto reductase family 1 member B11 antibody
    • Aldo-keto reductase family 1 member B10 (aldose reductase) antibody
    • Aldo-keto reductase family 1 member B10 antibody
    • Aldo-keto reductase family 1 member B11 (aldose reductase-like) antibody
    • aldose reductase like 1 antibody
    • Aldose reductase like antibody
    • aldose reductase like peptide antibody
    • Aldose reductase related protein antibody
    • Aldose reductase-like antibody
    • Aldose reductase-related protein antibody
    • ALDRLn antibody
    • ARL 1 antibody
    • ARL-1 antibody
    • ARL1 antibody
    • ARL1 Human small intestine reductase antibody
    • ARP antibody
    • hARP antibody
    • HIS antibody
    • HSI antibody
    • SI reductase antibody
    • Small intestine reductase antibody
    see all

Anti-AKR1B10 antibody images

  • All lanes : Anti-AKR1B10 antibody (ab96417) at 1/1000 dilution

    Lane 1 : MOLT4 whole cell lysate
    Lane 2 : Raji whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 36 kDa
  • ab96417, at 1/200 dilution, staining AKR1B10 in paraformaldehyde-fixed HeLa cells by Immunofluorescence. The lower image is merged with DNA probe.
  • ab96417, at 1/500 dilution, staining AKR1B10 in paraffin-embedded OVCAR3 xenograft sections by Immunohistochemistry.
  • ab96417 staining AKR1B10 in Human endometrial cancer tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5% serum for 60 minutes at 25°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/200) for 20 hours at 4°C. A Goat anti-rabbit IgG + IgM H+L polyclonal (1/100) was used as the secondary antibody.

    See Abreview

References for Anti-AKR1B10 antibody (ab96417)

This product has been referenced in:
  • Hevir N  et al. Decreased levels of AKR1B1 and AKR1B10 in cancerous endometrium compared to adjacent non-cancerous tissue. Chem Biol Interact : (2012). WB, IHC-P ; Human . Read more (PubMed: 23146748) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (endometrial cancer)
Specification endometrial cancer
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: sodium citrate buffer (pH=6) in microwave oven
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Feb 13 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (endometrial cancer and adjacent control endometriu)
Loading amount 30 µg
Specification endometrial cancer and adjacent control endometriu
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Feb 12 2013

Thank you for your inquiry.
I was in contact with the lab and unfortunately, we did not experimentally test if there is a cross reaction of the antibody to the other proteins.

In general, we do not expect any cross reactivity if the homo...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"