Recombinant
RabMAb

Anti-AKR1B10 antibody [EPR14421] (ab192865)

Overview

  • Product name
    Anti-AKR1B10 antibody [EPR14421]
    See all AKR1B10 primary antibodies
  • Description
    Rabbit monoclonal [EPR14421] to AKR1B10
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human AKR1B10 aa 100-200. The exact sequence is proprietary.
    Database link: O60218

  • Positive control
    • A549 and SW480 cell lysate; Human liver and stomach tissues; SW480 and A549 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab192865 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt 1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/50.

Target

  • Function
    Acts as all-trans-retinaldehyde reductase. Can efficiently reduce aliphatic and aromatic aldehydes, and is less active on hexoses (in vitro). May be responsible for detoxification of reactive aldehydes in the digested food before the nutrients are passed on to other organs.
  • Tissue specificity
    Found in many tissues. Highly expressed in small intestine, colon and adrenal gland.
  • Sequence similarities
    Belongs to the aldo/keto reductase family.
  • Cellular localization
    Lysosome. Secreted. Secreted through a lysosome-mediated non-classical pathway.
  • Information by UniProt
  • Database links
  • Alternative names
    • AK1BA_HUMAN antibody
    • AKR1B10 antibody
    • AKR1B11 antibody
    • AKR1B12 antibody
    • Aldo keto reductase family 1 member B10 antibody
    • aldo keto reductase family 1 member B11 antibody
    • Aldo-keto reductase family 1 member B10 (aldose reductase) antibody
    • Aldo-keto reductase family 1 member B10 antibody
    • Aldo-keto reductase family 1 member B11 (aldose reductase-like) antibody
    • aldose reductase like 1 antibody
    • Aldose reductase like antibody
    • aldose reductase like peptide antibody
    • Aldose reductase related protein antibody
    • Aldose reductase-like antibody
    • Aldose reductase-related protein antibody
    • ALDRLn antibody
    • ARL 1 antibody
    • ARL-1 antibody
    • ARL1 antibody
    • ARL1 Human small intestine reductase antibody
    • ARP antibody
    • hARP antibody
    • HIS antibody
    • HSI antibody
    • SI reductase antibody
    • Small intestine reductase antibody
    see all

Images

  • Anti-AKR1B10 antibody [EPR14421] (ab192865) at 1/10000 dilution + A549 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 36 kDa
    Observed band size: 36 kDa



    Blocking buffer and concentration: 5% NFDM/TBST

  • Anti-AKR1B10 antibody [EPR14421] (ab192865) at 1/1000 dilution + SW480 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 36 kDa
    Observed band size: 36 kDa



    Blocking buffer and concentration: 5% NFDM/TBST

  • Immunohistochemical analysis of paraffin embedded Human liver tissue labeling AKR1B10 with ab192865 at a 1/1000 dilution. A prediluted HRP Polymer for Rabbit IgG was used as the secondary antibody. Hematoxylin counterstain.

  • Immunohistochemical analysis of paraffin embedded human stomach tissue sections labeling AKR1B10 using ab192865 at a 1/1000 dilution. A prediluted HRP Polymer for Rabbit IgG was used as the secondary antibody. Hematoxylin counterstain.

  • Immunofluorescent analysis of 4% paraformaldehyde fixed SW480 cells labeling AKR1B10 with ab192865 at a 1/50 dilution. A Goat anti rabbit IgG (Alexa Fluor®488) (ab150077) was used as the secondary antibody at a 1/400 dilution. Counterstain DAPI. Cells were permeabilized using 0.1% Triton X-100.

  • Flow cytometric analysis of A549 cells labeling AKR1B10 using ab192865 at a 1/50 dilution (red). Goat anti rabbit IgG (FITC) was used as the secondary antibody at a 1/150 dilution. Isotype control Rabbit monoclonal IgG (black). Unlabeled control cells without incubation with primary antibody and secondary antibody (blue). Cells were fixed in 2% paraformaldehyde.

References

ab192865 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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