MitoSciences (MS1095)

Anti-AKR1C1/AKR1C2 [4B6AF3] antibody (ab131375)

Overview

  • Product nameAnti-AKR1C1/AKR1C2 [4B6AF3] antibodySee all AKR1C1/AKR1C2 primary antibodies ...
  • Description
    Mouse monoclonal [4B6AF3] to AKR1C1/AKR1C2
  • Tested applicationsWB, ICC/IF more details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Rabbit, Cow
  • Immunogen

    Purified protein cocktail.

  • Positive control
    • WB: HeLa, HepG2, HLH, HHH, MLH, RLH and RHH tissue samples ICC/IF: Hela cells

Properties

Applications

Our Abpromise guarantee covers the use of ab131375 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB Use a concentration of 2 µg/ml. Predicted molecular weight: 37 kDa.
ICC/IF Use a concentration of 5 µg/ml. (paraformaldehyde fixed cells)

Target

  • Database links
  • Alternative names
    • 2-dihydrobenzene-1 antibody
    • 2-diol dehydrogenase antibody
    • 20 alpha (3 alpha) hydroxysteroid dehydrogenase antibody
    • 20 ALPHA HSD antibody
    • 20 alpha hydroxysteroid dehydrogenase antibody
    • 20-alpha-HSD antibody
    • 20-alpha-hydroxysteroid dehydrogenase antibody
    • 20ALPHAHSD antibody
    • 2ALPHAHSD antibody
    • AK1C1 antibody
    • AK1C1_HUMAN antibody
    • AKR1C1 antibody
    • Aldo keto reductase family 1 member C1 antibody
    • Aldo-keto reductase family 1 member C1 antibody
    • C9 antibody
    • Chlordecone reductase homolog antibody
    • Chlordecone reductase homolog HAKRC antibody
    • DD1 antibody
    • DD1/DD2 antibody
    • DDH antibody
    • DDH1 antibody
    • Dihydrodiol dehydrogenase 1 antibody
    • Dihydrodiol dehydrogenase 1/2 antibody
    • dihydrodiol dehydrogenase isoform DD1 antibody
    • H37 antibody
    • HAKRC antibody
    • HBAB antibody
    • Hepatic dihydrodiol dehydrogenase antibody
    • High affinity hepatic bile acid-binding protein antibody
    • High-affinity hepatic bile acid-binding protein antibody
    • Indanol dehydrogenase antibody
    • MBAB antibody
    • MGC8954 antibody
    • Trans-1 antibody
    • Trans-1,2 dihydrobenzene 1,2 diol dehydrogenase antibody
    • Type II 3 alpha hydroxysteroid dehydrogenase antibody
    see all

Anti-AKR1C1/AKR1C2 [4B6AF3] antibody images

  • All lanes : Anti-AKR1C1/AKR1C2 [4B6AF3] antibody (ab131375) at 2 µg/ml

    Lane 1 : Molecular weight marker
    Lane 2 : HeLa Cell Lysate at 15 µg
    Lane 3 : HepG2 Cell Lysate at 15 µg
    Lane 4 : Human Liver Homogenate (HLH) at 10 µg
    Lane 5 : Human Heart Homogenate (HHH) at 10 µg
    Lane 6 : Mouse Liver Homogenate (MLH) at 10 µg
    Lane 7 : Rat Liver Homogenate (RLH) at 10 µg
    Lane 8 : Rat Heart Homogenate (RHH) at 10 µg

    Secondary
    Goat anti-Mouse AP at 1/3000 dilution

    Predicted band size : 37 kDa
  • Immunofluorescence analysis of AKR1C1 in HeLa cells, using ab131375 at 5 µg/ml.
    The cells were paraformaldehyde fixed (4%, 20 minutes) and Triton X-100 permeabilized (0.1%, 15 min). The cells were then incubated with ab131375 for 2h at room temperature or over night at 4°C. The secondary antibody was (red) Alexa Fluor® 594 Goat anti-Mouse IgG (H+L) used at a 1/1000 dilution for 1h. 1% BSA was used as the blocking agent for all blocking steps. The target protein locates to the cytosol.

References for Anti-AKR1C1/AKR1C2 [4B6AF3] antibody (ab131375)

ab131375 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"