Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab131375 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 µg/ml. Predicted molecular weight: 37 kDa.
ICC/IF Use a concentration of 5 µg/ml. (paraformaldehyde fixed cells)

Anti-AKR1C1/AKR1C2 antibody [4B6AF3] images

  • All lanes : Anti-AKR1C1/AKR1C2 antibody [4B6AF3] (ab131375) at 2 µg/ml

    Lane 1 : Molecular weight marker
    Lane 2 : HeLa Cell Lysate at 15 µg
    Lane 3 : HepG2 Cell Lysate at 15 µg
    Lane 4 : Human Liver Homogenate (HLH) at 10 µg
    Lane 5 : Human Heart Homogenate (HHH) at 10 µg
    Lane 6 : Mouse Liver Homogenate (MLH) at 10 µg
    Lane 7 : Rat Liver Homogenate (RLH) at 10 µg
    Lane 8 : Rat Heart Homogenate (RHH) at 10 µg

    Secondary
    Goat anti-Mouse AP at 1/3000 dilution

    Predicted band size : 37 kDa
  • Immunofluorescence analysis of AKR1C1 in HeLa cells, using ab131375 at 5 µg/ml.
    The cells were paraformaldehyde fixed (4%, 20 minutes) and Triton X-100 permeabilized (0.1%, 15 min). The cells were then incubated with ab131375 for 2h at room temperature or over night at 4°C. The secondary antibody was (red) Alexa Fluor® 594 Goat anti-Mouse IgG (H+L) used at a 1/1000 dilution for 1h. 1% BSA was used as the blocking agent for all blocking steps. The target protein locates to the cytosol.

References for Anti-AKR1C1/AKR1C2 antibody [4B6AF3] (ab131375)

This product has been referenced in:
  • Li C  et al. High-Content Functional Screening of AEG-1 and AKR1C2 for the Promotion of Metastasis in Liver Cancer. J Biomol Screen 21:101-7 (2016). Read more (PubMed: 26318406) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"