The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.01 - 0.1 µg/ml. Predicted molecular weight: 37 kDa.
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
FunctionCatalyzes the conversion of aldehydes and ketones to alcohols. Catalyzes the reduction of prostaglandin (PG) D2, PGH2 and phenanthrenequinone (PQ) and the oxidation of 9-alpha,11-beta-PGF2 to PGD2. Functions as a bi-directional 3-alpha-, 17-beta- and 20-alpha HSD. Can interconvert active androgens, estrogens and progestins with their cognate inactive metabolites. Preferentially transforms androstenedione (4-dione) to testosterone.
Tissue specificityExpressed in many tissues including adrenal gland, brain, kidney, liver, lung, mammary gland, placenta, small intestine, colon, spleen, prostate and testis. The dominant HSD in prostate and mammary gland. In the prostate, higher levels in epithelial cells than in stromal cells. In the brain, expressed in medulla, spinal cord, frontotemporal lobes, thalamus, subthalamic nuclei and amygdala. Weaker expression in the hippocampus, substantia nigra and caudate.
Sequence similaritiesBelongs to the aldo/keto reductase family.
ab115326, at 5µg/ml, staining AKR1C3 in Formalin-fixed, Paraffin-embedded Human Small intestine tissue by Immunohistochemistry followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Western blot - Anti-AKR1C3 antibody (ab115326)
Anti-AKR1C3 antibody (ab115326) at 0.03 µg/ml (incubated for 1 hour) + Human breast lysate (in RIPA buffer) at 35 µg Developed using the ECL technique
Predicted band size : 37 kDa
References for Anti-AKR1C3 antibody (ab115326)
has not yet been referenced specifically in any publications.
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