For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
ab126432 is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human, mouse and rat cell lysates. By determining phosphorylated Akt protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis. (UniprotID: P31749)
This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human, mouse and rat phospho-Akt (Ser473). An anti-pan Akt antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and Akt present in a sample is bound to the wells by the immobilized antibody. The wells are washed and anti-Akt (Ser473) antibody is used to detect phosphorylated Akt (Ser473). After washing away unbound antibody, HRP-conjugated anti-rabbit IgG is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Akt (Ser473) bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Get higher sensitivity in only 90 minutes with AKT 1/2/3 (pS473) ELISA Kit (ab176635) from our SimpleStep ELISA® range.
|Components||1 x 96 tests|
|20X Wash Buffer Concentrate||1 x 25ml|
|2x Cell lysate buffer||1 x 5ml|
|5X Assay Diluent||1 x 15ml|
|Akt Microplate (12 strips x 8 wells) coated with anti-pan Akt antibody||1 unit|
|Detection Antibody Akt (Ser473): rabbit anti-phospho-Akt (Ser473)||2 vials|
|HRP-conjugated anti-rabbit IgG||1 x 25µl|
|Positive Control: lyophilized powder from NIH3T3 cell lysate||1 vial|
|Stop Solution||1 x 8ml|
|TMB One-Step Substrate Reagent||1 x 12ml|
Our Abpromise guarantee covers the use of ab126432 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab126432 has not yet been referenced specifically in any publications.