Overview

  • Product nameAnti-AKT1 (phospho S473) antibodySee all AKT1 primary antibodies ...
  • Description
    Rabbit polyclonal to AKT1 (phospho S473)
  • SpecificityThe immunogen was raised against Human AKT1 phosphorylated at serine 473, however due to the high degree of similarity between AKT1, AKT2 and AKT3 the antibody is also predicted to react with all these isoforms.
  • Tested applicationsWB more details
  • Species reactivity
    Reacts with: Mouse, African Green Monkey
    Predicted to work with: Rat, Cow, Human, Xenopus laevis
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Human AKT, phosphorylated at S473.

    (Peptide available as ab18622.)

  • Positive control
    • This antibody gave a positive signal in NIH 3T3 treated with Vanadate and PDGF Whole Cell Lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab18206 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
  • Application notesWB: Use at a concentration of 1 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa). Can be blocked with AKT peptide - phospho S473 (ab18622).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionPlays a role as a key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation (By similarity). General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI(3)K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase. The activated form can suppress FoxO gene transcription and promote cell cycle progression. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly.
    • Tissue specificityExpressed in all human cell types so far analyzed. The Tyr-176 phosphorylated form shows a significant increase in expression in breast cancers during the progressive stages i.e. normal to hyperplasia (ADH), ductal carcinoma in situ (DCIS), invasive ductal carcinoma (IDC) and lymph node metastatic (LNMM) stages.
    • Involvement in diseaseDefects in AKT1 are a cause of susceptibility to breast cancer (BC) [MIM:114480]. A common malignancy originating from breast epithelial tissue. Breast neoplasms can be distinguished by their histologic pattern. Invasive ductal carcinoma is by far the most common type. Breast cancer is etiologically and genetically heterogeneous. Important genetic factors have been indicated by familial occurrence and bilateral involvement. Mutations at more than one locus can be involved in different families or even in the same case.
      Defects in AKT1 are associated with colorectal cancer (CRC) [MIM:114500].
      Defects in AKT1 are associated with susceptibility to ovarian cancer [MIM:604370]; also called susceptibility to familial breast-ovarian cancer type 1 (BROVCA1).
    • Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
      Contains 1 AGC-kinase C-terminal domain.
      Contains 1 PH domain.
      Contains 1 protein kinase domain.
    • DomainBinding of the PH domain to the phosphatidylinositol 3-kinase alpha (PI(3)K) results in its targeting to the plasma membrane. The PH domain mediates interaction with TNK2 and Tyr-176 is also essential for this interaction.
      The AGC-kinase C-terminal mediates interaction with THEM4.
    • Post-translational
      modifications
      Phosphorylation on Thr-308, Ser-473 and Tyr-474 is required for full activity. Activated TNK2 phosphorylates it on Tyr-176 resulting in its binding to the anionic plasma membrane phospholipid PA. This phosphorylated form localizes to the cell membrane, where it is targeted by PDPK1 and PDPK2 for further phosphorylations on Thr-308 and Ser-473 leading to its activation. Ser-473 phosphorylation by mTORC2 favors Thr-308 phosphorylation by PDPK1. Ser-473 phosphorylation is enhanced by interaction with AGAP2 isoform 2 (PIKE-A). Ser-473 phosphorylation is enhanced in focal cortical dysplasias with Taylor-type balloon cells.
      Ubiquitinated; undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination. TRAF6-induced 'Lys-63'-linked AKT1 ubiquitination is critical for phosphorylation and activation. When ubiquitinated, it translocates to the plasma membrane, where it becomes phosphorylated. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome.
    • Cellular localizationCytoplasm. Nucleus. Cell membrane. Nucleus after activation by integrin-linked protein kinase 1 (ILK1). Nuclear translocation is enhanced by interaction with TCL1A. Phosphorylation on Tyr-176 by TNK2 results in its localization to the cell membrane where it is targeted for further phosphorylations on Thr-308 and Ser-473 leading to its activation and the activated form translocates to the nucleus.
    • Target information above from: UniProt accession P31749 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
    • Alternative names
      • AKT 1 antibody
      • AKT 2 antibody
      • AKT 3 antibody
      • AKT antibody
      • AKT1 antibody
      • AKT1_HUMAN antibody
      • AKT2 antibody
      • AKT3 antibody
      • C AKT antibody
      • CAKT antibody
      • DKFZP434N0250 antibody
      • MGC99656 antibody
      • Murine thymoma viral (v akt) homolog 2 antibody
      • murine thymoma viral (v akt) oncogene homolog 1 antibody
      • Murine thymoma viral (vakt) oncogene homolog 1 antibody
      • PKB Akt antibody
      • PKB Akt antibody
      • PKB alpha antibody
      • PKB antibody
      • PKB beta antibody
      • PKB-ALPHA antibody
      • PKBalpha antibody
      • PKBalpha antibody
      • PKBBETA antibody
      • PKBG antibody
      • PRKBA antibody
      • PRKBA antibody
      • PRKBB antibody
      • PRKBG antibody
      • Protein kinase Akt 2 antibody
      • Protein kinase Akt 3 antibody
      • Protein Kinase B Alpha antibody
      • Protein kinase B antibody
      • Protein kinase B beta antibody
      • Protein kinase B gamma antibody
      • Proto-oncogene c-Akt antibody
      • RAC alpha antibody
      • RAC alpha serine/threonine protein kinase antibody
      • RAC antibody
      • RAC beta antibody
      • RAC beta serine/threonine protein kinase antibody
      • RAC gamma antibody
      • RAC gamma serine/threonine protein kinase antibody
      • RAC PK alpha antibody
      • RAC PK beta antibody
      • RAC PK gamma antibody
      • Rac protein kinase alpha antibody
      • Rac protein kinase beta antibody
      • RAC Serine/Threonine Protein Kinase antibody
      • RAC Serine/Threonine Protein Kinase antibody
      • RAC-alpha serine/threonine-protein kinase antibody
      • RAC-PK-alpha antibody
      • Serine threonine protein kinase Akt 3 antibody
      • STK 2 antibody
      • STK2 antibody
      • V AKT Murine Thymoma Viral Oncogene Homolog 1 antibody
      • V AKT Murine Thymoma Viral Oncogene Homolog 1 antibody
      • v akt murine thymoma viral oncogene homolog 2 antibody
      • v akt murine thymoma viral oncogene homolog 3 antibody
      • VAKT Murine Thymona Viral Oncogene 1 antibody
      see all

    Anti-AKT1 (phospho S473) antibody images

    • All lanes : Anti-AKT1 (phospho S473) antibody (ab18206) at 1 µg/ml

      Lane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
      Lane 2 : NIH 3T3 treated with Vanadate and PDGF Whole Cell Lysate
      Lane 3 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate with Human pan-AKT (phospho S473) peptide (ab18622) at 1 µg/ml
      Lane 4 : NIH 3T3 treated with Vanadate and PDGF Whole Cell Lysate with Human pan-AKT (phospho S473) peptide (ab18622) at 1 µg/ml
      Lane 5 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate with Human AKT1 (unmodified ) peptide (ab24649) at 1 µg/ml
      Lane 6 : NIH 3T3 treated with Vanadate and PDGF Whole Cell Lysate with Human AKT1 (unmodified ) peptide (ab24649) at 1 µg/ml

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Performed under reducing conditions.

      Predicted band size : 57 kDa
      Observed band size : 57-60 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 130 kDa,78 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 2 minutes

    References for Anti-AKT1 (phospho S473) antibody (ab18206)

    This product has been referenced in:
    • Taylor SY  et al. Folic acid modulates eNOS activity via effects on posttranslational modifications and protein-protein interactions. Eur J Pharmacol 714:193-201 (2013). WB . Read more (PubMed: 23796957) »
    • Witte F  et al. Negative regulation of Wnt signaling mediated by CK1-phosphorylated Dishevelled via Ror2. FASEB J 24:2417-26 (2010). WB ; African Green Monkey . Read more (PubMed: 20215527) »

    See all 2 Publications for this product

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