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Products:Neuroscience >> Neurology process >> Neural Signal Transduction
Overview
Product name
Anti-AKT1 (phospho S473) antibody
See all AKT1 products (62) ...
Description
Rabbit polyclonal to AKT1 (phospho S473)
Specificity
The region of AKT1 surrounding S473 has a high degree of similarity to the corresponding regions in AKT2 and AKT3 and thus may cross react with these proteins if phosphorylated on the corresponding serine residue.
Tested applications
IF, IHC-P, WBmore details
Cross reactivity
Reacts with
Cow, Human
Immunogen
Synthetic peptide: CHFPQFSpYSAS conjugated to KLH, corresponding to C terminal amino acids 468-477 of Human AKT.
CHFPQFSpYS AS
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
0.02M K Phosphate, 0.15M NaCl, 0.01% Sodium Azide, pH7.2
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Signal transduction >> Protein phosphorylation >> Serine/threonine kinases >> AKT
Epigenetics and Nuclear Signaling >> Cell cycle >> Apoptosis >> Nuclear
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> PKB / AKT
Neuroscience >> Neurology process >> Neural Signal Transduction
Applications
Show applications key
Our Abpromise guarantee covers the use of ab8932 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IF: Use at an assay dependent dilution.
IHC-P
IHC-P: 1/100IHC-P: 1/100
WB: Use at an assay dependent dilution. Predicted molecular weight: 56 kDa.
Target
Function
Plays a role as a key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation (By similarity). General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI(3)K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase. The activated form can suppress FoxO gene transcription and promote cell cycle progression. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly.
Tissue specificity
Expressed in all human cell types so far analyzed. The Tyr-176 phosphorylated form shows a significant increase in expression in breast cancers during the progressive stages i.e. normal to hyperplasia (ADH), ductal carcinoma in situ (DCIS), invasive ductal carcinoma (IDC) and lymph node metastatic (LNMM) stages.
Involvement in disease
Defects in AKT1 are a cause of susceptibility to breast cancer (BC) [MIM:114480]. A common malignancy originating from breast epithelial tissue. Breast neoplasms can be distinguished by their histologic pattern. Invasive ductal carcinoma is by far the most common type. Breast cancer is etiologically and genetically heterogeneous. Important genetic factors have been indicated by familial occurrence and bilateral involvement. Mutations at more than one locus can be involved in different families or even in the same case.
Defects in AKT1 are associated with colorectal cancer (CRC) [MIM:114500].
Defects in AKT1 are associated with susceptibility to ovarian cancer [MIM:604370]; also called susceptibility to familial breast-ovarian cancer type 1 (BROVCA1).
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 PH domain.
Contains 1 protein kinase domain.
Domain
Binding of the PH domain to the phosphatidylinositol 3-kinase alpha (PI(3)K) results in its targeting to the plasma membrane. The PH domain mediates interaction with TNK2 and Tyr-176 is also essential for this interaction.
The AGC-kinase C-terminal mediates interaction with THEM4.
Post-translational
modifications
Phosphorylation on Thr-308, Ser-473 and Tyr-474 is required for full activity. Activated TNK2 phosphorylates it on Tyr-176 resulting in its binding to the anionic plasma membrane phospholipid PA. This phosphorylated form localizes to the cell membrane, where it is targeted by PDPK1 and PDPK2 for further phosphorylations on Thr-308 and Ser-473 leading to its activation. Ser-473 phosphorylation by mTORC2 favors Thr-308 phosphorylation by PDPK1. Ser-473 phosphorylation is enhanced by interaction with AGAP2 isoform 2 (PIKE-A). Ser-473 phosphorylation is enhanced in focal cortical dysplasias with Taylor-type balloon cells.
Ubiquitinated; undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination. TRAF6-induced 'Lys-63'-linked AKT1 ubiquitination is critical for phosphorylation and activation. When ubiquitinated, it translocates to the plasma membrane, where it becomes phosphorylated. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome.
Cellular localization
Cytoplasm. Nucleus. Cell membrane. Nucleus after activation by integrin-linked protein kinase 1 (ILK1). Nuclear translocation is enhanced by interaction with TCL1A. Phosphorylation on Tyr-176 by TNK2 results in its localization to the cell membrane where it is targeted for further phosphorylations on Thr-308 and Ser-473 leading to its activation and the activated form translocates to the nucleus.
Target information above from: UniProt accessionP31749
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- C AKT antibody
- AKT 1 antibody
- AKT1 antibody
see all
Database links
- Entrez Gene: 280991 Cow
- Entrez Gene: 207 Human
- Omim: 164730 Human
- SwissProt: Q01314 Cow
- SwissProt: P31749 Human
- Unigene: 525622 Human
Anti-AKT1 (phospho S473) antibody images:
- AKT1 (phospho S473) antibody (ab8932)
Normal breast tissue. Akt is weakly phosphorylated in normal tissue in the breast. The phosphorylated Akt is clearly localised in the cytoplasm.
The phopho Ser 473 Akt antibody (ab8932) is used with no pretreatment at a dilution of 1:100 using Dako techmate streptavidin-biotin reagents.
- AKT (phospho S473) antibody (ab8932)
Akt is phosphorylated on Ser 473 in breast tumor. The staining is much stronger than the weak basal level of phosphorylation in normal breast. The staining is cytoplasmic, as in normal tissue.
The phopho Ser 473 Akt antibody (ab8932) is used with no pretreatment at a dilution of 1:100 using Dako techmate streptavidin-biotin reagents.
This image is a magnification of the accompanying breast tumor image.
Western blot - Anti-AKT1 (phospho S473) antibody (ab8932)
Predicted band size : 56 kDa
ab8932 at a 1:200 dilution in Western Blot staining nuclear extract from cells infected with adenovirus expressing nuclear-targeted Akt kinase.
Immunocytochemistry/ Immunofluorescence - AKT1 (phospho S473) antibody (ab8932)
Confocal image of ab8932 at a 1:40 dilution staining cardiomyocytes infected with wild-type Akt in conjunction with a texas-red conjugated phalloidin to label filamentous actin in the cardiomyocytes.
Immunocytochemistry/ Immunofluorescence - AKT1 (phospho S473) antibody (ab8932)
Confocal image of ab8932 at a 1:40 dilution staining cardiomyocytes infected with wild-type Akt in conjunction with a texas-red conjugated phalloidin to label filamentous actin in the cardiomyocytes.
- AKT1 (phospho S473) antibody (ab8932)
Akt is phosphorylated on Ser 473 in breast tumor. The staining is much stronger than the weak basal level of phosphorylation in normal breast. The staining is cytoplasmic, as in normal tissue.
The phopho Ser 473 Akt antibody (ab8932) is used with no pretreatment at a dilution of 1:100 using Dako techmate streptavidin-biotin reagents.
References for Anti-AKT1 (phospho S473) antibody (ab8932)
This product has been referenced in:
- Cima Iet al. Cancer genetics-guided discovery of serum biomarker signatures for diagnosis and prognosis of prostate cancer. Proc Natl Acad Sci U S A 108:3342-7 (2011). IHC; Human.Read more (PubMed: 21300890) »
- Dupont Jet al. FSH-stimulated PTEN activity accounts for the lack of FSH mitogenic effect in prepubertal rat Sertoli cells. Mol Cell Endocrinol 315:271-6 (2010).Read more (PubMed: 19778579) »
See all 6 publications for this product
Publishing research using ab8932? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"