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- AKT1 (phospho S473) antibody (ab8932)
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- AKT (phospho S473) antibody (ab8932)
(enlarge)
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- AKT1 (phospho S473) antibody (ab8932)
(enlarge)
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Product Name
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AKT1 (phospho S473) antibody
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See all AKT1 antibodies (55)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to AKT1 (phospho S473)
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Immunogen
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Synthetic peptide: CHFPQFSpYSAS conjugated to KLH, corresponding to C terminal amino acids 468-477 of Human AKT.
BLAST 'CHFPQFSpYSAS' with  or 
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Reacts with
(species key)
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Hu
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Specificity
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The region of AKT1 surrounding S473 has a high degree of similarity to the corresponding regions in AKT2 and AKT3 and thus may cross react with these proteins if phosphorylated on the corresponding serine residue.
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Tested applications
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ICC/IF, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IF: Use at an assay dependent dilution.
IHC-P: 1/100.
WB: Use at an assay dependent dilution. Predicted molecular weight: 56 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Cytoplasmic, and nuclear after activation by integrin linked protein kinase 1 (ILK1).
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Research areas
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Cancer >> Signal transduction >> Protein phosphorylation >> Serine/threonine kinases >> AKT
Chromatin and Nuclear Signaling >> Cell cycle >> Apoptosis >> Nuclear
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> PKB / AKT
Neuroscience >> Neurology process >> Neural Signal Transduction
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Relevance
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The serine threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet derived growth factor. The activation is rapid and specific. In the developing nervous system AKT is a critical mediator of growth factor induced neuronal survival. Survival factors can suppress apoptosis in a transcription independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene (referenced from entrez gene).
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AKT1 (phospho S473) antibody (ab8932) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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0.02M K Phosphate, 0.15M NaCl, 0.01% Sodium Azide, pH7.2 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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This product was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.
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Form
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Liquid
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Concentration
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Batch dependent within range: 0.75 - 1.00 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this AKT1 (phospho S473) antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for AKT1 (phospho S473) antibody images, references, products related to ab8932 and other tools.
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AKT1 (phospho S473) antibody images:
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 - AKT1 (phospho S473) antibody (ab8932)
Normal breast tissue. Akt is weakly phosphorylated in normal tissue in the breast. The phosphorylated Akt is clearly localised in the cytoplasm.
The phopho Ser 473 Akt antibody (ab8932) is used with no pretreatment at a dilution of 1:100 using Dako techmate streptavidin-biotin reagents.
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- AKT (phospho S473) antibody (ab8932)
Akt is phosphorylated on Ser 473 in breast tumor. The staining is much stronger than the weak basal level of phosphorylation in normal breast. The staining is cytoplasmic, as in normal tissue.
The phopho Ser 473 Akt antibody (ab8932) is used with no pretreatment at a dilution of 1:100 using Dako techmate streptavidin-biotin reagents.
This image is a magnification of the accompanying breast tumor image.
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- AKT1 (phospho S473) antibody (ab8932)
Predicted band size : 56 kDa
ab8932 at a 1:200 dilution in Western Blot staining nuclear extract from cells infected with adenovirus expressing nuclear-targeted Akt kinase.
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Immunocytochemistry/ Immunofluorescence - AKT1 (phospho S473) antibody (ab8932)
Confocal image of ab8932 at a 1:40 dilution staining cardiomyocytes infected with wild-type Akt in conjunction with a texas-red conjugated phalloidin to label filamentous actin in the cardiomyocytes.
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Immunocytochemistry/ Immunofluorescence - AKT1 (phospho S473) antibody (ab8932)
Confocal image of ab8932 at a 1:40 dilution staining cardiomyocytes infected with wild-type Akt in conjunction with a texas-red conjugated phalloidin to label filamentous actin in the cardiomyocytes.
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- AKT1 (phospho S473) antibody (ab8932)
Akt is phosphorylated on Ser 473 in breast tumor. The staining is much stronger than the weak basal level of phosphorylation in normal breast. The staining is cytoplasmic, as in normal tissue.
The phopho Ser 473 Akt antibody (ab8932) is used with no pretreatment at a dilution of 1:100 using Dako techmate streptavidin-biotin reagents.
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References for AKT1 (phospho S473) antibody (ab8932)
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This product has been used in: (two most recent references)
Shah T et al. Epidermal growth factor receptor expression and activation in neuroendocrine tumours. J Neuroendocrinol 18:355-60 (2006). PubMed: 16629834
Volanti C et al. Distinct transduction mechanisms of cyclooxygenase 2 gene activation in tumour cells after photodynamic therapy. Oncogene 24:2981-91 (2005). PubMed: 15735712
If you publish research using ab8932 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to AKT1
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AKT1 (phospho S473) antibody - more information
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Price and availability of products related to AKT1 (phospho S473) antibody |
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AKT1 (phospho S473) antibody - related products:
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AKT1 (phospho S473) antibody - other tools:
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Contact Abcam with a Technical Enquiry about AKT1 (phospho S473) antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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