Overview

  • Product nameAnti-AKT1/2 antibodySee all AKT1/2 primary antibodies ...
  • Description
    Rabbit polyclonal to AKT1/2
  • Tested applicationsWB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (Human) conjugated to KLH

  • Positive control
    • WB: mouse or Rat brain lysate; HeLa or Hs-67 cell lysate. IHC-P: heart, prostate or thymus tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab109870 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 56 kDa.
IP Use at an assay dependent dilution.
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • RelevanceThe serine/threonine kinase AKT (protein kinase B or PKB) has a central role in the regulation of several signaling pathways controlling cell proliferation, apoptosis, angiogenesis, and diabetes. In humans, there are three genes in the "AKT family": AKT1, AKT2, and AKT3. AKT1 is catalytically inactive in serum starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet derived growth factor. The activation is rapid and specific. In the developing nervous system AKT is a critical mediator of growth factor induced neuronal survival. Survival factors can suppress apoptosis in a transcription independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. AKT2 is a putative oncogene and is a general protein kinase capable of phophorylating several known proteins. AKT2 is amplified and overexpressed in some human carcinomas. AKT2 acts primarily as a regulator of glucose metabolism.
  • Cellular localizationATK1: Cytoplasm. Nucleus. Cell membrane. Note: Nucleus after activation by integrin-linked protein kinase 1 (ILK1). Nuclear translocation is enhanced by interaction with TCL1A.
  • Database links
  • Alternative names
    • AKT 1 antibody
    • AKT 2 antibody
    • AKT antibody
    • AKT1 antibody
    • AKT1 kinase antibody
    • AKT2 antibody
    • AKT2 kinase antibody
    • C AKT antibody
    • MGC99656 antibody
    • Murine thymoma viral (v akt) homolog 2 antibody
    • Oncogene AKT1 antibody
    • PKB alpha antibody
    • PKB antibody
    • PKB beta antibody
    • PKBB antibody
    • PKBBETA antibody
    • PRKBA antibody
    • PRKBB antibody
    • Protein kinase Akt 2 antibody
    • Protein Kinase B Alpha antibody
    • Protein kinase B antibody
    • Protein kinase B beta antibody
    • RAC antibody
    • RAC alpha antibody
    • RAC alpha serine/threonine protein kinase antibody
    • RAC BETA antibody
    • RAC beta serine threonine protein kinase antibody
    • RAC PK alpha antibody
    • RAC PK beta antibody
    • Rac protein kinase alpha antibody
    • Rac protein kinase beta antibody
    • RACbeta antibody
    • v akt murine thymoma viral oncogene homolog 1 antibody
    • v akt murine thymoma viral oncogene homolog 2 antibody
    see all

Anti-AKT1/2 antibody images

  • ab109870 at 10 µg/ml staining AKT1/2 in formalin-fixed, paraffin-embedded Human heart tissue after heat induced antigen retrieval in pH 6.0 citrate buffer.
    After incubation with the primary antibody, slides were incubated with a biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
  • ab109870 at 10 µg/ml staining AKT1/2 in formalin-fixed, paraffin-embedded Human placenta (red blood cells) after heat induced antigen retrieval in pH 6.0 citrate buffer.
    After incubation with the primary antibody, slides were incubated with a biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
  • ab109870 at 10 µg/ml staining AKT1/2 in formalin-fixed, paraffin-embedded Human prostate tissue after heat induced antigen retrieval in pH 6.0 citrate buffer.
    After incubation with the primary antibody, slides were incubated with a biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
  • ab109870 at 10 µg/ml staining AKT1/2 in formalin-fixed, paraffin-embedded Human thymus tissue after heat induced antigen retrieval in pH 6.0 citrate buffer.
    After incubation with the primary antibody, slides were incubated with a biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.

References for Anti-AKT1/2 antibody (ab109870)

ab109870 has not yet been referenced specifically in any publications.

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