Overview

  • Product nameAnti-AKT1/2 antibodySee all AKT1/2 primary antibodies ...
  • Description
    Rabbit polyclonal to AKT1/2
  • Specificityab6076 recognizes both AKT1 and AKT2. The sequence used as the immunogen differs from the mouse sequence by 1 amino acid.
  • Tested applicationsICC, WB, IHC, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to C terminal amino acids of Human AKT1/2.

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: HepG2; MCF7; HeLa; NIH3T3.

Properties

Applications

Our Abpromise guarantee covers the use of ab6076 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC 1/300.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 56 kDa).
IHC Use at an assay dependent dilution.
IP Use at an assay dependent dilution.
IHC-P Use at an assay dependent concentration.

Target

  • RelevanceThe serine/threonine kinase AKT (protein kinase B or PKB) has a central role in the regulation of several signaling pathways controlling cell proliferation, apoptosis, angiogenesis, and diabetes. In humans, there are three genes in the "AKT family": AKT1, AKT2, and AKT3. AKT1 is catalytically inactive in serum starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet derived growth factor. The activation is rapid and specific. In the developing nervous system AKT is a critical mediator of growth factor induced neuronal survival. Survival factors can suppress apoptosis in a transcription independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. AKT2 is a putative oncogene and is a general protein kinase capable of phophorylating several known proteins. AKT2 is amplified and overexpressed in some human carcinomas. AKT2 acts primarily as a regulator of glucose metabolism.
  • Cellular localizationATK1: Cytoplasm. Nucleus. Cell membrane. Note: Nucleus after activation by integrin-linked protein kinase 1 (ILK1). Nuclear translocation is enhanced by interaction with TCL1A.
  • Database links
  • Alternative names
    • AKT 1 antibody
    • AKT 2 antibody
    • AKT antibody
    • AKT1 antibody
    • AKT1 kinase antibody
    • AKT2 antibody
    • AKT2 kinase antibody
    • C AKT antibody
    • MGC9965 antibody
    • MGC99656 antibody
    • Murine thymoma viral (v akt) homolog 2 antibody
    • Murine thymoma viral (vakt) oncogene homolog 1 antibody
    • Oncogene AKT1 antibody
    • PKB Akt antibody
    • PKB alpha antibody
    • PKB antibody
    • PKB beta antibody
    • PKBalpha antibody
    • PKBB antibody
    • PKBBETA antibody
    • PRKBA antibody
    • PRKBB antibody
    • Protein kinase Akt 2 antibody
    • Protein Kinase B Alpha antibody
    • Protein kinase B antibody
    • Protein kinase B beta antibody
    • RAC alpha antibody
    • RAC alpha serine/threonine protein kinase antibody
    • RAC antibody
    • RAC BETA antibody
    • RAC beta serine threonine protein kinase antibody
    • RAC PK alpha antibody
    • RAC PK beta antibody
    • Rac protein kinase alpha antibody
    • Rac protein kinase beta antibody
    • RACbeta antibody
    • v akt murine thymoma viral oncogene homolog 1 antibody
    • v akt murine thymoma viral oncogene homolog 2 antibody
    • vAKT Murine Thymoma Viral Oncogene Homolog 1 antibody
    • VAKT Murine Thymona Viral Oncogene 1 antibody
    see all

Anti-AKT1/2 antibody images

  • ab6076 detecting strong nuclear staining of AKT1/2 in adult dorsal root ganglion cells cultured for 2 days. ab6076 was used at 1/300 (incubated for 2h at room temperature). Secondary antibody used is anti-rabbit alexa fluor 488 (at 1/1000 for 1hr at room temperature). The cell diameter is approx. 25µm. Nucl = Nucleus; Cyt.=Cytoplasm.
  • All lanes : Anti-AKT1/2 antibody (ab6076) at 1 µg/ml

    Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 4 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 56 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)


    Exposure time : 2 minutes

References for Anti-AKT1/2 antibody (ab6076)

This product has been referenced in:
  • Shen R  et al. The biological features of PanIN initiated from oncogenic Kras mutation in genetically engineered mouse models. Cancer Lett 339:135-43 (2013). WB . Read more (PubMed: 23887057) »
  • Riaz A  et al. Receptor-specific mechanisms regulate phosphorylation of AKT at Ser473: role of RICTOR in ß1 integrin-mediated cell survival. PLoS One 7:e32081 (2012). IP ; Human . Read more (PubMed: 22384145) »

See all 4 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Tongue)
Specification Tongue
Fixative Bouin's solution
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris-EDTA pH: 9
Permeabilization No
Blocking step Casein in sodium carbonate buffer, pH 9,6 as blocking agent for 30 minute(s) · Concentration: 0.2% · Temperature: 22°C
Username

Dr. Tonny Petersen

Verified customer

Submitted Dec 27 2012

Thank you for contacting us.

All the products you shortlisted seem to be suitable for your work, however, looking at the availability of those products, I would recommend the following one that are currently in stock:
Anti-AKT1 (N-term)ant...

Read More

Dear Daniel, Thank you for your enquiry. Some researchers refer to AKT1 as AKT, but because of the multiple splice variants the AKT1 is the preferred nomenclature. ab6076 has not been found to cross react with the other AKT variants. I hop...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"