Overview

  • Product nameAnti-AKT1/2/3 antibodySee all AKT1/2/3 primary antibodies ...
  • Description
    Rabbit polyclonal to AKT1/2/3
  • Specificityab79360 detects endogenous levels of total AKT1/2/3 protein.
  • Tested applicationsWB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Rat, Human, Culex pipiens
    Predicted to work with: Mouse
  • Immunogen

    Synthesized non-phosphopeptide derived from human AKT1/2/3 around the phosphorylation site of tyrosine 315/316/312 (P-E-YP-L-A).

  • Positive control
    • Human brain tissue and A549 cell extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab79360 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 56 kDa).
IHC-P 1/50 - 1/100.
ELISA 1/40000.

Target

  • FunctionIGF-1 leads to the activation of AKT3, which may play a role in regulating cell survival. Capable of phosphorylating several known proteins. Truncated isoform 2/PKB gamma 1 without the second serine phosphorylation site could still be stimulated but to a lesser extent.
  • Tissue specificityIn adult tissues, it is highly expressed in brain, lung and kidney, but weakly in heart, testis and liver. In fetal tissues, it is highly expressed in heart, liver and brain and not at all in kidney.
  • Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 PH domain.
    Contains 1 protein kinase domain.
  • DomainBinding of the PH domain to the phosphatidylinositol 3-kinase alpha (PI(3)K) results in its targeting to the plasma membrane.
  • Post-translational
    modifications
    Phosphorylation on Thr-305 and Ser-472 is required for full activity (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
    Ubiquitinated. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome.
  • Cellular localizationCytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation.
  • Information by UniProt
  • Database links
  • Alternative names
    • AKT antibody
    • AKT1 antibody
    • AKT1 kinase antibody
    • AKT2 antibody
    • AKT2 kinase antibody
    • Akt3 antibody
    • AKT3_HUMAN antibody
    • CAKT antibody
    • DKFZp434N0250 antibody
    • kinase Akt1 antibody
    • MGC99656 antibody
    • Murine thymoma viral (v-akt) homolog 2 antibody
    • PKB antibody
    • PKB gamma antibody
    • PKB-GAMMA antibody
    • PKB/Akt antibody
    • PKBALPHA antibody
    • PKBB antibody
    • PKBBETA antibody
    • PKBG antibody
    • PKBGAMMA antibody
    • PRKBA antibody
    • PRKBB antibody
    • PRKBG antibody
    • Protein kinase Akt-3 antibody
    • Protein kinase B antibody
    • Protein kinase B gamma antibody
    • RAC antibody
    • rac protein kinase alpha antibody
    • rac protein kinase beta antibody
    • RAC-gamma antibody
    • RAC-gamma serine/threonine-protein kinase antibody
    • RAC-PK-gamma antibody
    • RACALPHA antibody
    • RACalpha serine/threonine kinase antibody
    • RACBETA antibody
    • RACgamma antibody
    • RACgamma serine/threonine protein kinase antibody
    • RACPKgamma antibody
    • serine threonine protein kinase antibody
    • STK-2 antibody
    • STK2 antibody
    • thymoma viral proto oncogene 1 antibody
    • thymoma viral proto oncogene antibody
    • vakt murine thymoma viral oncogene homolog 1 antibody
    • vakt murine thymoma viral oncogene homolog 2 antibody
    • vakt murine thymoma viral oncogene homolog 3 antibody
    see all

Anti-AKT1/2/3 antibody images

  • All lanes : Anti-AKT1/2/3 antibody (ab79360) at 1/500 dilution

    Lane 1 : A549 cell extract at 5 µg
    Lane 2 : A549 cell extract with immunizing peptide at 5 µg


    Predicted band size : 56 kDa
    Observed band size : 48 kDa (why is the actual band size different from the predicted?)
  • ab79360, at 1/50 dilution, staining AKT1/2/3 in human brain tissue by Immunohistochemistry in the presence (right) or absence (left) of immunizing peptide. Formalin-fixed, paraffin-embedded section.

References for Anti-AKT1/2/3 antibody (ab79360)

This product has been referenced in:
  • Han JW  et al. Impaired PI3K/Akt signal pathway and hepatocellular injury in high-fat fed rats. World J Gastroenterol 16:6111-8 (2010). WB ; Rat . Read more (PubMed: 21182226) »

See 1 Publication for this product

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (10)
Sample Culex pipiens Tissue lysate - whole (whole fly homogenate)
Specification whole fly homogenate
Treatment blood feeding with sheep blood
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Jiawen Xu

Verified customer

Submitted Aug 05 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"