Overview

  • Product nameAnti-AKT1/2/3 antibodySee all AKT1/2/3 primary antibodies ...
  • Description
    Rabbit polyclonal to AKT1/2/3
  • Specificityab82538 recognizes AKT1, AKT2, and AKT3.
  • Tested applicationsWB more details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide, corresponding to amino acids 471-475 of Human AKT1/2/3

  • Positive control
    • Human recombinant AKT1, AKT2, and AKT3; HeLa, HEK-293T, Jurkat, THP1, and 3T6 cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab82538 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB 1/1000. Predicted molecular weight: 56 kDa.

Target

  • FunctionIGF-1 leads to the activation of AKT3, which may play a role in regulating cell survival. Capable of phosphorylating several known proteins. Truncated isoform 2/PKB gamma 1 without the second serine phosphorylation site could still be stimulated but to a lesser extent.
  • Tissue specificityIn adult tissues, it is highly expressed in brain, lung and kidney, but weakly in heart, testis and liver. In fetal tissues, it is highly expressed in heart, liver and brain and not at all in kidney.
  • Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 PH domain.
    Contains 1 protein kinase domain.
  • DomainBinding of the PH domain to the phosphatidylinositol 3-kinase alpha (PI(3)K) results in its targeting to the plasma membrane.
  • Post-translational
    modifications
    Phosphorylation on Thr-305 and Ser-472 is required for full activity (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
    Ubiquitinated. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome.
  • Cellular localizationCytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation.
  • Target information above from: UniProt accession Q9Y243 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • AKT antibody
    • AKT1 antibody
    • AKT1 kinase antibody
    • AKT2 antibody
    • AKT2 kinase antibody
    • AKT3 antibody
    • AKT3_HUMAN antibody
    • CAKT antibody
    • DKFZp434N0250 antibody
    • kinase Akt1 antibody
    • MGC99656 antibody
    • Murine thymoma viral (v-akt) homolog 2 antibody
    • PKB antibody
    • PKB gamma antibody
    • PKB-GAMMA antibody
    • PKB/Akt antibody
    • PKBALPHA antibody
    • PKBB antibody
    • PKBBETA antibody
    • PKBG antibody
    • PKBGAMMA antibody
    • PRKBA antibody
    • PRKBB antibody
    • PRKBG antibody
    • Protein kinase Akt-3 antibody
    • Protein kinase B antibody
    • Protein kinase B gamma antibody
    • RAC antibody
    • rac protein kinase alpha antibody
    • rac protein kinase beta antibody
    • RAC-gamma antibody
    • RAC-gamma serine/threonine-protein kinase antibody
    • RAC-PK-gamma antibody
    • RACALPHA antibody
    • RACalpha serine/threonine kinase antibody
    • RACBETA antibody
    • RACgamma antibody
    • RACgamma serine/threonine protein kinase antibody
    • RACPKgamma antibody
    • serine threonine protein kinase antibody
    • STK-2 antibody
    • STK2 antibody
    • thymoma viral proto oncogene 1 antibody
    • thymoma viral proto oncogene antibody
    • vakt murine thymoma viral oncogene homolog 1 antibody
    • vakt murine thymoma viral oncogene homolog 2 antibody
    • vakt murine thymoma viral oncogene homolog 3 antibody
    see all

Anti-AKT1/2/3 antibody images

  • All lanes : Anti-AKT1/2/3 antibody (ab82538) at 1/1000 dilution

    Lane 1 : human recombinant AKT1
    Lane 2 : human recombinant AKT2
    Lane 3 : human recombinant AKT3

    Lysates/proteins at 0.02 µg per lane.


    Predicted band size : 56 kDa
    Observed band size : 85 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-AKT1/2/3 antibody (ab82538) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : HEK-293T cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : THP1 cell lysate
    Lane 5 : 3T6 cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 56 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)

References for Anti-AKT1/2/3 antibody (ab82538)

ab82538 has not yet been referenced specifically in any publications.

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