Overview

  • Product nameAnti-AKT1/3 antibodySee all AKT1/3 primary antibodies ...
  • Description
    Rabbit polyclonal to AKT1/3
  • SpecificityThe antibody detects endogenous levels of total AKT1/3 protein
  • Tested applicationsWB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthesized non-phosphopeptide derived from human AKT1/3 around the phosphorylation site of tyrosine 437/434 (T-R-YP-F-D).

  • Positive control
    • A549 cell extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab79361 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
ELISA 1/10000.

Target

  • RelevanceAKT1 and AKT3 are members of the AKT, also called PKB, serine/threonine protein kinase family. AKT kinases are known to be regulators of cell signaling in response to insulin and growth factors. They are involved in a wide variety of biological processes including cell proliferation, differentiation, apoptosis, tumorigenesis, as well as glycogen synthesis and glucose uptake. This kinase has been shown to be stimulated by platelet derived growth factor (PDGF), insulin, and insulin like growth factor 1 (IGF1). Alternatively splice transcript variants encoding distinct isoforms have been described. The protein kinase encoded by the AKT1 gene is catalytically inactive in serum starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3 kinase. In the developing nervous system AKT is a critical mediator of growth factor induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene
  • Cellular localizationCell Membrane, Cytoplasmic and Nuclear
  • Database links
  • Alternative names
    • AKT antibody
    • AKT1 antibody
    • AKT1 kinase antibody
    • AKT3 antibody
    • AKT3 kinase antibody
    • CAKT antibody
    • murine thymoma viral (vakt) oncogene homolog 1 antibody
    • murine thymoma viral (vakt) oncogene homolog 3 antibody
    • ONCOGENE AKT1 antibody
    • PKB antibody
    • PKBAkt antibody
    • PKBALPHA antibody
    • PKBG antibody
    • PKBGAMMA antibody
    • PRKBA antibody
    • PRKBG antibody
    • protein kinase B antibody
    • protein kinase B gamma antibody
    • PROTEIN KINASE BALPHA antibody
    • RAC antibody
    • rac protein kinase alpha antibody
    • RAC protein kinase alpha RACPK alpha antibody
    • RACALPHA antibody
    • RACalpha serine/threonine protein kinase antibody
    • RACgamma antibody
    • RACgamma serine/threonine protein kinase antibody
    • RACPK gamma antibody
    • serine/threonine protein kinase antibody
    • STK2 antibody
    • thymoma viral protooncogene 1 antibody
    • thymoma viral protooncogene 3 antibody
    • thymoma viral protooncogene antibody
    • vakt murine thymoma viral oncogene like protein 1 antibody
    see all

Anti-AKT1/3 antibody images

  • All lanes : Anti-AKT1/3 antibody (ab79361) at 1/500 dilution

    Lane 1 : A549 cell extract
    Lane 2 : A549 cell extract with immunizing peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 56 kDa
    Observed band size : 56 kDa
    Additional bands at : 75 kDa. We are unsure as to the identity of these extra bands.

References for Anti-AKT1/3 antibody (ab79361)

ab79361 has not yet been referenced specifically in any publications.

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