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Western blot - AKT2 antibody (ab13991)
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Product Name
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AKT2 antibody
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See all AKT2 antibodies (16)...
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Product type
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Primary antibodies
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Description
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Chicken polyclonal to AKT2
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Immunogen
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Synthetic peptide: NSLKQRAPGEDPMDYKC, corresponding to amino acids 108-124 of Human AKT2.
BLAST 'NSLKQRAPGEDPMDYKC' with  or 
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Reacts with
(species key)
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Hu
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Tested applications
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IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: 1/500. Predicted molecular weight: 56 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Cytoplasmic
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Research areas
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Cardiovascular >> Heart >> Cardiac metabolism
Cardiovascular >> Atherosclerosis >> Diabetes associated
Cancer >> Signal transduction >> Protein phosphorylation >> Serine/threonine kinases >> AKT
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> PKB / AKT
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Relevance
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The serine/threonine kinase Akt (protein kinase B or PKB) has a central role in the regulation of several signaling pathways controlling cell proliferation, apoptosis, angiogenesis, and diabetes. In humans, there are three genes in the "Akt family": Akt1, Akt2, and Akt3.
AKT2 is a putative oncogene and is a general protein kinase capable of phophorylating several known proteins. AKT2 is amplified and overexpressed in some human carcinomas. AKT2 acts primarily as a regulator of glucose metabolism.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Chicken
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Clonality
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Polyclonal
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Isotype
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IgY
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.01% Sodium Azide
Constituents: PBS, pH 7.7 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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Peptide immunogen affinity column.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this AKT2 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for AKT2 antibody images, references, products related to ab13991 and other tools.
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AKT2 antibody images:
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 Western blot - AKT2 antibody (ab13991)
AKT2 antibody (ab13991) at 1/500 dilution + mouse skin, whole tissue lysate at 4 µg
Secondary
Goat polyclonal to Mouse IgG - H&L (HRP) (ab6789) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 56 kDa
Observed band size : 58 kDa (why is the actual band size different from the predicted?)
Exposure time : 1 minute This image is courtesy of an anonymous Abreview
See Abreview
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References for AKT2 antibody (ab13991)
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This product has been used in:
O'Shaughnessy RF et al. AKT-dependent HspB1 (Hsp27) activity in epidermal differentiation. J Biol Chem 282:17297-305 (2007). PubMed: 17439945
If you publish research using ab13991 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to AKT2
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AKT2 antibody - more information
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Customer reviews (feedback) regarding AKT2 antibody |
Customer FAQs regarding AKT2 antibody |
Protocols for AKT2 antibody |
Price and availability of products related to AKT2 antibody |
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AKT2 antibody - related products:
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AKT2 antibody - other tools:
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Contact Abcam with a Technical Enquiry about AKT2 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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