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Products:Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> PKB / AKT
Overview
Product name
Anti-AKT2 antibody
See all AKT2 products (16) ...
Description
Chicken polyclonal to AKT2
Tested applications
WB, IHC-Pmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat
Immunogen
Synthetic peptide: NSLKQRAPGEDPMDYKC, corresponding to amino acids 108-124 of Human AKT2.
NSLKQRAPGE DPMDYKC
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: PBS, pH 7.7
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Peptide immunogen affinity column.
Clonality
Polyclonal
Isotype
IgY
Research areas
Cardiovascular >> Heart >> Cardiac metabolism
Cardiovascular >> Atherosclerosis >> Diabetes associated
Cancer >> Signal transduction >> Protein phosphorylation >> Serine/threonine kinases >> AKT
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> PKB / AKT
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Western blot - AKT2 antibody (ab13991)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab13991 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
WB: 1/500. Predicted molecular weight: 56 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
General protein kinase capable of phosphorylating several known proteins.
Tissue specificity
Expressed in all human cell types so far analyzed.
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 PH domain.
Contains 1 protein kinase domain.
Post-translational
modifications
Phosphorylation on Thr-309 and Ser-474 is required for full activity.
Ubiquitinated; undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination. TRAF6-induced 'Lys-63'-linked AKT2 ubiquitination. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome.
Target information above from: UniProt accessionP31751
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AKT 2 antibody
- Akt2 antibody
- AKT2_HUMAN antibody
see all
Database links
- Entrez Gene: 208 Human
- Entrez Gene: 11652 Mouse
- Entrez Gene: 25233 Rat
- Omim: 164731 Human
- SwissProt: P31751 Human
- SwissProt: Q60823 Mouse
- SwissProt: P47197 Rat
- Unigene: 631535 Human
see all
Anti-AKT2 antibody images:
Western blot - AKT2 antibody (ab13991)
Anti-AKT2 antibody (ab13991) at 1/500 dilution + mouse skin, whole tissue lysate at 4 µg
Secondary
Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) (ab6789) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 56 kDa
Observed band size : 58 kDa (why is the actual band size different from the predicted?)
Exposure time : 1 minute
This image is courtesy of an anonymous Abreview
References for Anti-AKT2 antibody (ab13991)
This product has been referenced in:
- O'Shaughnessy RFet al. AKT-dependent HspB1 (Hsp27) activity in epidermal differentiation. J Biol Chem 282:17297-305 (2007).Read more (PubMed: 17439945) »
See 1 publication for this product
Publishing research using ab13991? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"