Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab13991 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
  • Application notesWB: 1/500. Predicted molecular weight: 56 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionGeneral protein kinase capable of phosphorylating several known proteins.
    • Tissue specificityExpressed in all human cell types so far analyzed.
    • Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
      Contains 1 AGC-kinase C-terminal domain.
      Contains 1 PH domain.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications
      Phosphorylation on Thr-309 and Ser-474 is required for full activity.
      Ubiquitinated; undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination. TRAF6-induced 'Lys-63'-linked AKT2 ubiquitination. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome.
    • Information by UniProt
    • Database links
    • Alternative names
      • Akt2 antibody
      • AKT2_HUMAN antibody
      • HIHGHH antibody
      • murine thymoma viral (v-akt) homolog-2 antibody
      • PKB antibody
      • PKB beta antibody
      • PKBB antibody
      • PKBBETA antibody
      • PRKBB antibody
      • Protein kinase Akt 2 antibody
      • Protein kinase Akt-2 antibody
      • Protein kinase B beta antibody
      • rac protein kinase beta antibody
      • RAC-BETA antibody
      • RAC-beta serine/threonine-protein kinase antibody
      • RAC-PK-beta antibody
      • v akt murine thymoma viral oncogene homolog 2 antibody
      see all

    Anti-AKT2 antibody images

    • Anti-AKT2 antibody (ab13991) at 1/500 dilution + mouse skin, whole tissue lysate at 4 µg

      Secondary
      Goat Anti-Mouse IgG H&L (HRP) (ab6789) at 1/3000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 56 kDa
      Observed band size : 58 kDa (why is the actual band size different from the predicted?)


      Exposure time : 1 minute

      This image is courtesy of an anonymous Abreview

      See Abreview

    References for Anti-AKT2 antibody (ab13991)

    This product has been referenced in:

    See all 3 Publications for this product

    Product Wall

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (Mouse epidermal squamous cell carcinoma section)
    Specification Mouse epidermal squamous cell carcinoma section
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated
    Permeabilization No
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 23°C
    Username

    Abcam user community

    Verified customer

    Submitted Jul 15 2008

    Application Immunohistochemistry (Frozen sections)
    Sample Human Tissue sections (papilloma basal cell region)
    Specification papilloma basal cell region
    Fixative Formaldehyde
    Permeabilization Yes - Triton X
    Blocking step Fish Skin Gelatin as blocking agent for 1 hour(s) and 1 minute(s) · Concentration: 0.8% · Temperature: 23°C
    Username

    Abcam user community

    Verified customer

    Submitted Jun 26 2008

    Application Western blot
    Sample Mouse Tissue lysate - whole (Skin)
    Loading amount 4 µg
    Specification Skin
    Gel Running Conditions Reduced Denaturing (10%)
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Username

    Abcam user community

    Verified customer

    Submitted Jun 25 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"