Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab13991 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
  • Application notes
    WB: 1/500. Predicted molecular weight: 56 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      General protein kinase capable of phosphorylating several known proteins.
    • Tissue specificity
      Expressed in all human cell types so far analyzed.
    • Sequence similarities
      Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
      Contains 1 AGC-kinase C-terminal domain.
      Contains 1 PH domain.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications
      Phosphorylation on Thr-309 and Ser-474 is required for full activity.
      Ubiquitinated; undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination. TRAF6-induced 'Lys-63'-linked AKT2 ubiquitination. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome.
    • Information by UniProt
    • Database links
    • Alternative names
      • Akt2 antibody
      • AKT2_HUMAN antibody
      • HIHGHH antibody
      • murine thymoma viral (v-akt) homolog-2 antibody
      • PKB antibody
      • PKB beta antibody
      • PKBB antibody
      • PKBBETA antibody
      • PRKBB antibody
      • Protein kinase Akt 2 antibody
      • Protein kinase Akt-2 antibody
      • Protein kinase B beta antibody
      • rac protein kinase beta antibody
      • RAC-BETA antibody
      • RAC-beta serine/threonine-protein kinase antibody
      • RAC-PK-beta antibody
      • v akt murine thymoma viral oncogene homolog 2 antibody
      see all

    Anti-AKT2 antibody images

    • Anti-AKT2 antibody (ab13991) at 1/500 dilution + mouse skin, whole tissue lysate at 4 µg
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 56 kDa
      Observed band size : 58 kDa (why is the actual band size different from the predicted?)


      Exposure time : 1 minute

      This image is courtesy of an anonymous Abreview

      See Abreview

    References for Anti-AKT2 antibody (ab13991)

    This product has been referenced in:

    See all 3 Publications for this product

    Product Wall

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Mouse epidermal squamous cell carcinoma section)
    Antigen retrieval step
    Heat mediated
    Permeabilization
    No
    Specification
    Mouse epidermal squamous cell carcinoma section
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 23°C
    Fixative
    Formaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Jul 15 2008

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (papilloma basal cell region)
    Specification
    papilloma basal cell region
    Fixative
    Formaldehyde
    Permeabilization
    Yes - Triton X
    Blocking step
    Fish Skin Gelatin as blocking agent for 1 hour(s) and 1 minute(s) · Concentration: 0.8% · Temperature: 23°C
    Username

    Abcam user community

    Verified customer

    Submitted Jun 26 2008

    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Skin)
    Gel Running Conditions
    Reduced Denaturing (10%)
    Loading amount
    4 µg
    Specification
    Skin
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Username

    Abcam user community

    Verified customer

    Submitted Jun 25 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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