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Anti-AKT2 (phospho S474) antibody (ab97727)


  • Product nameAnti-AKT2 (phospho S474) antibody
    See all AKT2 primary antibodies
  • Description
    Rabbit polyclonal to AKT2 (phospho S474)
  • Specificityab97727 detects endogenous levels of AKT2 only when phosphorylated at serine 474. The region of AKT2 surrounding S474 has a high degree of similarity to the corresponding regions in AKT1 and AKT3 and thus may cross react with these proteins if phosphorylated on the corresponding serine residue.
  • Tested applicationsIHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic phosphopeptide derived from Human AKT2 around the phosphorylation site of serine 474 (Q-F-SP-Y-S).

  • Positive control
    • Human lung carcinoma tissue; 293 cell extracts, treated with serum.



Our Abpromise guarantee covers the use of ab97727 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100.
WB 1/500 - 1/1000. Predicted molecular weight: 56 kDa.


  • FunctionGeneral protein kinase capable of phosphorylating several known proteins.
  • Tissue specificityExpressed in all human cell types so far analyzed.
  • Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 PH domain.
    Contains 1 protein kinase domain.
  • Post-translational
    Phosphorylation on Thr-309 and Ser-474 is required for full activity.
    Ubiquitinated; undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination. TRAF6-induced 'Lys-63'-linked AKT2 ubiquitination. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome.
  • Information by UniProt
  • Database links
  • Alternative names
    • Akt2 antibody
    • AKT2_HUMAN antibody
    • HIHGHH antibody
    • murine thymoma viral (v-akt) homolog-2 antibody
    • PKB antibody
    • PKB beta antibody
    • PKBB antibody
    • PKBBETA antibody
    • PRKBB antibody
    • Protein kinase Akt 2 antibody
    • Protein kinase Akt-2 antibody
    • Protein kinase B beta antibody
    • rac protein kinase beta antibody
    • RAC-BETA antibody
    • RAC-beta serine/threonine-protein kinase antibody
    • RAC-PK-beta antibody
    • v akt murine thymoma viral oncogene homolog 2 antibody
    see all

Anti-AKT2 (phospho S474) antibody images

  • ab97727 at 1/50 dilution staining AKT2 in Human lung carcinoma by Immunohistochemistry, Paraffin-embedded tissue in the absence or presence of the immunising peptide.
  • All lanes : Anti-AKT2 (phospho S474) antibody (ab97727) at 1/500 dilution

    Lane 1 : 293 cell extracts, untreated
    Lane 2 : 293 cell extracts, treated with serum

    Predicted band size : 56 kDa

References for Anti-AKT2 (phospho S474) antibody (ab97727)

ab97727 has not yet been referenced specifically in any publications.

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As far as we know the antibody ab97727 has never been tested in IP, it is therefore not covered by our Abpromise guarantee.
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Expiration date: September 15th, 2012

I am very pleased to hear you would like to accept our offer and test ab97727for reactivity with AKT1. This code will give you: 1 free primary antibody before the expiration ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Mammary cell)
Loading amount 250 µg
Specification Mammary cell
Treatment TGF beta for 6hrs
Gel Running Conditions Reduced Denaturing (10)
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

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Submitted Oct 08 2011