The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. PubMed: 19679212
1/250 - 1/1000.
Application notesIs unsuitable for Flow Cyt.
Cellular localizationAKT3: Cytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation.
AKT1: Cytoplasm. Nucleus. Cell membrane. Nucleus after activation by integrin-linked protein kinase 1 (ILK1). Nuclear translocation is enhanced by interaction with TCL1A. Phosphorylation on Tyr-176 by TNK2 results in its localization to the cell membrane where it is targeted for further phosphorylations on Thr-308 and Ser-473 leading to its activation and the activated form translocates to the nucleus.
Western blot - Anti-AKT3 + AKT1 antibody [E45] (ab32038)
All lanes : Anti-AKT3 + AKT1 antibody [E45] (ab32038) at 1/10000 dilution
Lane 1 : GFP tagged AKT1 transfected 293T cell lysate Lane 2 : Empty vector transfected 293T cell lysate Lane 3 : GFP tagged AKT2 transfected 293T cell lysate Lane 4 : Empty vector transfected 293T cell lysate Lane 5 : GFP tagged AKT3 transfected 293T cell lysate Lane 6 : Empty vector transfected 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Exposure time : 1 second
Blocking/Diluting buffer: 5% NFDM/TBST.
Observed band: 82kDa, 56kDa.
82kDa band represents GFP tagged AKT. 56kDa band represents endogenous AKT.
Anti-AKT3 + AKT1 antibody [E45] (ab32038)
Immunohistochemistry (Paraffin-embedded sections) analysis using ab32038 at a dilution of 1/1000 - 1/1250 and squamous lung carcinomaImmunohistochemistry (Paraffin-embedded sections) analysis using ab32038 at a dilution of 1/1000 - 1/1250 and squamous lung carcinoma
References for Anti-AKT3 + AKT1 antibody [E45] (ab32038)
This product has been referenced in:
Feng M et al. Therapeutically targeting glypican-3 via a conformation-specific single-domain antibody in hepatocellular carcinoma. Proc Natl Acad Sci U S A110:E1083-91 (2013).
Read more (PubMed: 23471984) »
Ulici V et al. The role of Akt1 in terminal stages of endochondral bone formation: angiogenesis and ossification. Bone45:1133-45 (2009).
Read more (PubMed: 19679212) »