Overview

  • Product nameAnti-AKT3 + AKT1 antibody [E45]
    See all AKT3+AKT1 primary antibodies
  • Description
    Rabbit monoclonal [E45] to AKT3 + AKT1
  • Specificityab32038 detects Akt1 and Akt3.
  • Tested applicationsSuitable for: WB, IHC-P, ICC, IPmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow
  • Immunogen

    Synthetic peptide corresponding to Human AKT1 aa 450-550.
    Database link: P31749

  • Positive control
    • Squamous lung carcinoma
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab32038 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. PubMed: 19679212
IHC-P 1/250 - 1/1000.
ICC 1/1000.
IP 1/100.
  • Application notesIs unsuitable for Flow Cyt.
  • Target

    Anti-AKT3 + AKT1 antibody [E45] images

    • All lanes : Anti-AKT3 + AKT1 antibody [E45] (ab32038) at 1/10000 dilution

      Lane 1 : GFP tagged AKT1 transfected 293T cell lysate
      Lane 2 : Empty vector transfected 293T cell lysate
      Lane 3 : GFP tagged AKT2 transfected 293T cell lysate
      Lane 4 : Empty vector transfected 293T cell lysate
      Lane 5 : GFP tagged AKT3 transfected 293T cell lysate
      Lane 6 : Empty vector transfected 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Exposure time : 1 second

      Blocking/Diluting buffer: 5% NFDM/TBST.

      Observed band: 82kDa, 56kDa.

      82kDa band represents GFP tagged AKT. 56kDa band represents endogenous AKT. 

    • Immunohistochemistry (Paraffin-embedded sections) analysis using ab32038 at a dilution of 1/1000 - 1/1250 and squamous lung carcinoma

    References for Anti-AKT3 + AKT1 antibody [E45] (ab32038)

    This product has been referenced in:
    • Chen YC  et al. High mobility group box 1-induced epithelial mesenchymal transition in human airway epithelial cells. Sci Rep 6:18815 (2016). WB ; Human . Read more (PubMed: 26739898) »
    • Zhang Z  et al. Upregulated periostin promotes angiogenesis in keloids through activation of the ERK 1/2 and focal adhesion kinase pathways, as well as the upregulated expression of VEGF and angiopoietin-1. Mol Med Rep 11:857-64 (2015). WB ; Human . Read more (PubMed: 25369801) »

    See all 4 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"