The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. PubMed: 19679212
1/250 - 1/1000.
Application notesIs unsuitable for Flow Cyt.
Cellular localizationAKT3: Cytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation.
AKT1: Cytoplasm. Nucleus. Cell membrane. Nucleus after activation by integrin-linked protein kinase 1 (ILK1). Nuclear translocation is enhanced by interaction with TCL1A. Phosphorylation on Tyr-176 by TNK2 results in its localization to the cell membrane where it is targeted for further phosphorylations on Thr-308 and Ser-473 leading to its activation and the activated form translocates to the nucleus.
Immunohistochemistry (Paraffin-embedded sections) analysis using ab32038 at a dilution of 1/1000 - 1/1250 and squamous lung carcinoma
References for Anti-AKT3 + AKT1 antibody [E45] (ab32038)
This product has been referenced in:
Chen YC et al. High mobility group box 1-induced epithelial mesenchymal transition in human airway epithelial cells. Sci Rep6:18815 (2016).
Read more (PubMed: 26739898) »
Feng M et al. Therapeutically targeting glypican-3 via a conformation-specific single-domain antibody in hepatocellular carcinoma. Proc Natl Acad Sci U S A110:E1083-91 (2013).
Read more (PubMed: 23471984) »