Overview

  • Product nameAnti-ALAD antibody
    See all ALAD primary antibodies
  • Description
    Rabbit polyclonal to ALAD
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Saccharomyces cerevisiae, Drosophila melanogaster, Zebrafish
  • Immunogen

    A region within synthetic peptide: SVMSYSAKFA SCFYGPFRDA AKSSPAFGDR RCYQLPPGAR GLALRAVDRD, corresponding to amino acids 192-241 of Human ALAD

  • Positive control
    • Jurkat cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab59013 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.25 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionCatalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen.
  • PathwayPorphyrin metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 1/4.
  • Involvement in diseaseDefects in ALAD are the cause of acute hepatic porphyria (AHP) [MIM:612740]. AHP is a form of porphyria. Porphyrias are inherited defects in the biosynthesis of heme, resulting in the accumulation and increased excretion of porphyrins or porphyrin precursors. They are classified as erythropoietic or hepatic, depending on whether the enzyme deficiency occurs in red blood cells or in the liver. AHP is characterized by attacks of gastrointestinal disturbances, abdominal colic, paralysis, and peripheral neuropathy. Most attacks are precipitated by drugs, alcohol, caloric deprivation, infections, or endocrine factors.
  • Sequence similaritiesBelongs to the ALADH family.
  • Information by UniProt
  • Database links
  • Alternative names
    • ALAD antibody
    • ALADH antibody
    • ALADR antibody
    • Aminolevulinate dehydratase antibody
    • Aminolevulinate, delta, dehydratase antibody
    • Delta aminolevulinic acid dehydratase antibody
    • Delta-aminolevulinic acid dehydratase antibody
    • HEM2_HUMAN antibody
    • Lv antibody
    • MGC5057 antibody
    • OTTMUSP00000009376 antibody
    • OTTMUSP00000009377 antibody
    • PBGS antibody
    • Porphobilinogen synthase antibody
    • RP11-10I9.1 antibody
    • RP24-189G18.9 antibody
    see all

Anti-ALAD antibody images

  • All lanes : Anti-ALAD antibody (ab59013) at 0.25 µg/ml

    Lane 1 : Molecular weight markers
    Lane 2 : Jurkat cell lysate at 10 µg

    Secondary
    Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution


    Predicted band size : 37 kDa
    Observed band size : 37 kDa

References for Anti-ALAD antibody (ab59013)

ab59013 has not yet been referenced specifically in any publications.

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