Alanine Transaminase Activity Assay Kit (Colorimetric/Fluorometric) (ab105134)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate reader
- Assay time: 1 hr 20 min
- Sample type: Cell culture media, Cell Lysate, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
- Sensitivity: 10 mU/well
Overview
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Product name
Alanine Transaminase Activity Assay Kit (Colorimetric/Fluorometric)
See all Alanine Transaminase kits -
Detection method
Colorimetric/Fluorometric -
Sample type
Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate, Cell culture media -
Assay type
Enzyme activity (quantitative) -
Sensitivity
> 10 mU/well -
Assay time
1h 20m -
Species reactivity
Reacts with: Mammals -
Product overview
Alanine Transaminase Activity Assay Kit (Colorimetric/Fluorometric) ab105134 is a rapid and simple assay used to quantify alanine transaminase (ALT) activity in mammalian samples.
In the ALT assay protocol, ALT transfers an amino group from alanine to α-ketoglutarate; producing pyruvate and glutamate. The pyruvate is detected in a reaction that converts a nearly colorless probe to a form that is colored (ODmax = 570 nm) and fluorescent (Ex/Em = 535/587 nm).
The kit has a detection limit of 10 mU per well.
ALT assay protocol summary:
- add samples and standards to wells
- add reaction mix and incubate for 10 min at 37ºC
- analyze every 2-3 min for 60 min with microplate reader in kinetic mode at 37ºC -
Notes
This product is manufactured by BioVision, an Abcam company and was previously called K752 Alanine Aminotransferase (ALT or SGPT) Activity Colorimetric/Fluorometric Assay Kit. K752-100 is the same size as the 100 test size of ab105134.
Alanine transaminase is also called alanine aminotransferase or serum glutamic pyruvic transaminase (ALT, ALAT, SGPT).
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests 2000 tests ALT Positive Control 1 vial 20 vials ALT Substrate Mix 1 vial 20 vials Assay Buffer XIII 1 x 25ml 20 x 25ml Development Enzyme Mix I 1 vial 20 vials OxiRed Probe 1 x 200µl 20 x 200µl Pyruvate Standard 1 x 100µl 20 x 100µl -
Research areas
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Alternative names
- Alanine Aminotransferase
- ALT
Images
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Liver samples from high fat diet (HFD) and standard carbohydrate diet (CHD) BALB/c and C57BL6/J mice were homogenised in an ALT assay buffer for the determination of ALT activity using ab105134. A separate batch of liver extracts was prepared and incubated in a buffer containing NP40 (5%) and supernatants containing the triglycerides were separated. Triglycerides concentration was determined on the supernatant fraction using ab65336. ALT activity and triglycerides concentration were determined by measuring OD at 570nm.
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Colorimetric standard curve: mean of duplicates (+/- SD) with background reads subtracted.
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Serum aspartate transaminase (AST) levels were measured using ab105135 and alanine transaminase (ALT) levels were measured using ab105134. Both levels were measured at various time periods post Con A injection. Mean values ± SD are shown (n = 4). ∗P < 0.05 and ∗∗P < 0.01.
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Fluorometric standard curve: mean of duplicates (+/- SD) with background reads subtracted.
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Alanine transaminase measured in mouse tissue lysates showing quantity (mU) per mg of tested sample.
Protein concentration for samples varied from 4 mg/mL to 13 mg/mL. Samples were diluted 9-27 fold and measured colorimetrically.
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Pyruvate measured colorimetrically in cell lysate after 20 min and 40 min incubation time showing quantity (nmol) per 1 mln of tested cells.
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Measurement of alanine transaminase in HepG2 cells (10 μg) and liver lysate (15 μg).
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Pyruvate measured fluorometrically in cell lysate after 20 min and 40 min incubation time showing quantity (nmol) per 1 mln of tested cells.
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Pyruvate measured in biological fluids after 20 min and 40 min incubation time showing quantity (nmol) per ml of tested sample.
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Pyruvate measured in mouse tissue lysates after 20 min and 40 min incubation time showing quantity (nmol) per mg of tested sample.
Datasheets and documents
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SDS download
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Datasheet download
References (123)
ab105134 has been referenced in 123 publications.
- Humpton TJ et al. p53-mediated redox control promotes liver regeneration and maintains liver function in response to CCl4. Cell Death Differ 29:514-526 (2022). PubMed: 34628485
- Xiao K et al. Potential roles of vitamin D binding protein in attenuating liver injury in sepsis. Mil Med Res 9:4 (2022). PubMed: 35057868
- Cicatiello AG et al. Thyroid hormone regulates glutamine metabolism and anaplerotic fluxes by inducing mitochondrial glutamate aminotransferase GPT2. Cell Rep 38:110409 (2022). PubMed: 35196498
- Farooqi HMU et al. Study of the Anticancer Potential of Plant Extracts Using Liver Tumor Microphysiological System. Life (Basel) 12:N/A (2022). PubMed: 35207423
- Liu G et al. Hepatic RACK1 deficiency protects against fulminant hepatitis through myeloid-derived suppressor cells. Theranostics 12:2248-2265 (2022). PubMed: 35265209