Alanine Transaminase Activity Assay Kit (Colorimetric/Fluorometric) (ab105134)

Overview

  • Product nameAlanine Transaminase Activity Assay Kit (Colorimetric/Fluorometric)
  • Tests
    100 x 1 assay
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell culture media
  • Assay typeQuantitative
  • Sensitivity
    > 10 mU/well
  • Species reactivity
    Reacts with: Species independent
  • Product overview

    Alanine Transaminase Activity Assay Kit (Colorimetric/Fluorometric) (ab105134) is a rapid and simple assay where alanine transminase (ALT) catalyzes the transfer of an amino group from alanine to a-ketoglutarate, the products of this reversible transamination reaction being pyruvate and glutamate. The pyruvate is detected in a reaction that concomitantly converts a nearly colorless probe to both color (ODmax = 570 nm) and fluorescence (Ex/Em = 535/587 nm). The kit provides a rapid, simple, sensitive, and reliable test suitable for high throughput activity assay of ALT with a detection limit of 10 mU per well.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Alanine transaminase, also called alanine aminotransferase or serum glutamic pyruvic transaminase (ALT, ALAT, SGPT, EC 2.6.1.2) is a transaminase enzyme found in serum and in various bodily tissues, but usually associated with the liver. It catalyzes the reaction: a-ketoglutarate + alanine ? glutamate + pyruvate. It is commonly measured clinically as a part of a diagnostic liver function test to determine liver health. Diagnostically, it is almost always measured in units/liter (U/L).

  • Tested applicationsSuitable for: Functional Studiesmore details

Properties

    Applications

    Our Abpromise guarantee covers the use of ab105134 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Functional Studies Use at an assay dependent dilution.

    Alanine Transaminase Activity Assay Kit (Colorimetric/Fluorometric) images

    • Colorimetric standard curve: mean of duplicates (+/- SD) with background reads subtracted.
    • Fluorometric standard curve: mean of duplicates (+/- SD) with background reads subtracted.

    • Alanine transaminase measured in mouse tissue lysates showing quantity (mU) per mg of tested sample.

      Protein concentration for samples varied from 4 mg/mL to 13 mg/mL. Samples were diluted 9-27 fold and measured colorimetrically.

    • Pyruvate measured colorimetrically in cell lysate after 20 min and 40 min incubation time showing quantity (nmol) per 1 mln of tested cells.

    • Measurement of alanine transaminase in HepG2 cells (10 μg) and liver lysate (15 μg).

    • Pyruvate measured fluorometrically in cell lysate after 20 min and 40 min incubation time showing quantity (nmol) per 1 mln of tested cells.

    • Pyruvate measured in biological fluids after 20 min and 40 min incubation time showing quantity (nmol) per ml of tested sample.

    • Pyruvate measured in mouse tissue lysates after 20 min and 40 min incubation time showing quantity (nmol) per mg of tested sample.

    Protocols

    References for Alanine Transaminase Activity Assay Kit (Colorimetric/Fluorometric) (ab105134)

    This product has been referenced in:
    • Serrao EM  et al. MRI with hyperpolarised [1-13C]pyruvate detects advanced pancreatic preneoplasia prior to invasive disease in a mouse model. Gut 65:465-75 (2016). Read more (PubMed: 26347531) »
    • Li J  et al. Tumour necrosis factor-a promotes liver ischaemia-reperfusion injury through the PGC-1a/Mfn2 pathway. J Cell Mol Med 18:1863-73 (2014). Functional Studies . Read more (PubMed: 24898700) »

    See all 3 Publications for this product

    Product Wall

    J’ai eu une réponse du laboratoire qui m’a confirmé que ce kit peut être utilisé sur des échantillons humain.

    Malheureusement le laboratoire n’a pas de conversion entre l’activit&eacut...

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    Ich möchte hiermit auch bestätigen, dass beide Kits für Zellkulturüberstand getestet und auch garantiert sind.

    Sie haben Recht, dass eine Probenaufbereitung nicht gesondert erwähnt wird.

    Zellkultur...

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    I am sorry for the delay in getting back to you. I now have some further information to share with you.

    Contrary to what I mentioned in my previous email, the L-Alanine Assay Kit (ab83394) is unlikely to be of use in the experiments you are pe...

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    Thank you for contacting us yesterday in regards to the Alanine Transaminase Activity Assay Kit (ab105134). I have now had a chance to have a look at this product to see if it would be suitable to use in your experiment to assess the transaminase inhib...

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    Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

    I would like to reassure you that this kit is tested and covered by our 6 month guarantee for. In the ...

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    Thank you for contacting us.

    You are correct, the first readin should be done at RT after 10 minutes. After this you can incubate at 37 degrees away from light before taking the second reading. We have not tested this product using RO water ...

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    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this h...

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    Thank you for your inquiry.

    I heard back from the lab that whole blood cannot be used with this assay kit as the color will disrupt the reading. You will have to get plasma/serum to use it.

    I hope this information helps. Please cont...

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    The consensus is that your sample prep and storage should be fine, though we do not have activity data for this enzyme for similar storage conditions.

    Please let me know if you have any other questions or concerns.

    Thank you for contacting us.

    The developer of the kit tells us that samples can be stored frozen in the assay buffer that is included with the kit (25 ml). I think his assumption is that you will prepare homogenates of the tissue in the buff...

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    1-10 of 16 Abreviews or Q&A

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"