Anti-Alanine Transaminase antibody (ab34832)

Overview

  • Product name
    Anti-Alanine Transaminase antibody
    See all Alanine Transaminase primary antibodies
  • Description
    Sheep polyclonal to Alanine Transaminase
  • Specificity
    Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Alanine Transaminase (ALT) [Pig Heart]. Cross reactivity against Alanine Transaminase (ALT) from other tissues may occur but have not been specifically determined.
  • Tested applications
    Suitable for: Dot blot, WB, ELISA, IPmore details
  • Species reactivity
    Reacts with: Pig
  • Immunogen

    Full length protein from Pig heart.

Properties

Applications

Our Abpromise guarantee covers the use of ab34832 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot
WB
ELISA
IP
  • Application notes
    Dot: Use at an assay dependent dilution.
    ELISA: 1/1000 - 1/5000. This product has been assayed against 1.0 ug of Alanine Transaminase (ALT) [Pig Heart] in a standard sandwich ELISA using Peroxidase conjugated affinity purified anti-Sheep IgG [H&L] (Goat) and ABTS as a substrate for 30 minutes at RT.
    IP: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 54 kDa.

    Suitable for most immunological methods requiring high titer and specificity.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles.
    • Tissue specificity
      Liver, kidney, heart, and skeletal muscles. Expressed at moderate levels in the adipose tissue.
    • Pathway
      Amino-acid degradation; L-alanine degradation via transaminase pathway; pyruvate from L-alanine: step 1/1.
    • Sequence similarities
      Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily.
    • Cellular localization
      Cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • AAT1 antibody
      • Alanine aminotransferase 1 antibody
      • Alanine aminotransferase antibody
      • ALAT1_HUMAN antibody
      • ALT1 antibody
      • Glutamate pyruvate transaminase 1 antibody
      • Glutamic alanine transaminase 1 antibody
      • Glutamic pyruvate transaminase (alanine aminotransferase) antibody
      • Glutamic pyruvic transaminase 1 antibody
      • Glutamic--alanine transaminase 1 antibody
      • Glutamic--pyruvic transaminase 1 antibody
      • GPT 1 antibody
      • gpt antibody
      • GPT1 antibody
      see all

    References

    ab34832 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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