The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Dot: Use at an assay dependent dilution.
ELISA: 1/1000 - 1/5000. This product has been assayed against 1.0 ug of Alanine Transaminase (ALT) [Pig Heart] in a standard sandwich ELISA using Peroxidase conjugated affinity purified anti-Sheep IgG [H&L] (Goat) and ABTS as a substrate for 30 minutes at RT.
IP: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 54 kDa.
Suitable for most immunological methods requiring high titer and specificity.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles.
Liver, kidney, heart, and skeletal muscles. Expressed at moderate levels in the adipose tissue.
Amino-acid degradation; L-alanine degradation via transaminase pathway; pyruvate from L-alanine: step 1/1.
Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily.