Anti-Alanine Transaminase antibody (ab59667)

Overview

  • Product nameAnti-Alanine Transaminase antibody
    See all Alanine Transaminase primary antibodies
  • Description
    Rabbit polyclonal to Alanine Transaminase
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow
  • Immunogen

    A region within synthetic peptide: LMGQRPITFL RQVLALCVNP DLLSSPNFPD DAKKRAERIL QACGGHSLGA, corresponding to N terminal amino acids 73-122 of Human Alanine Transaminase

  • Positive control
    • Jurkat cell lysate (WB) Human kidney tissue (IHC)

Properties

Applications

Our Abpromise guarantee covers the use of ab59667 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 4 - 8 µg/ml.
WB Use a concentration of 2.5 µg/ml. Predicted molecular weight: 54 kDa.Can be blocked with Human Alanine Transaminase peptide (ab90531). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionCatalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles.
  • Tissue specificityLiver, kidney, heart, and skeletal muscles. Expressed at moderate levels in the adipose tissue.
  • PathwayAmino-acid degradation; L-alanine degradation via transaminase pathway; pyruvate from L-alanine: step 1/1.
  • Sequence similaritiesBelongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • AAT1 antibody
    • Alanine aminotransferase 1 antibody
    • Alanine aminotransferase antibody
    • ALAT1_HUMAN antibody
    • ALT1 antibody
    • Glutamate pyruvate transaminase 1 antibody
    • Glutamic alanine transaminase 1 antibody
    • Glutamic pyruvate transaminase (alanine aminotransferase) antibody
    • Glutamic pyruvic transaminase 1 antibody
    • Glutamic--alanine transaminase 1 antibody
    • Glutamic--pyruvic transaminase 1 antibody
    • GPT 1 antibody
    • gpt antibody
    • GPT1 antibody
    see all

Anti-Alanine Transaminase antibody images

  • Lane 1 : marker
    Lane 2 : Anti-Alanine Transaminase antibody (ab59667) at 2.5 µg/ml

    Lane 1 : As above
    Lane 2 : Jurkat cell lysate at 10 µg with 5% skim milk / PBS buffer

    Secondary
    Lane 2 : HRP-conjugated anti-Rabbit IgG at 1/50000 dilution


    Predicted band size : 54 kDa
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using ab59667 at a dilution of 4-8 µg/ml, positively labelling epithelial cells of the renal tubule (indicated with arrows). Magnification 400X

References for Anti-Alanine Transaminase antibody (ab59667)

ab59667 has not yet been referenced specifically in any publications.

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