Anti-Alanyl-tRNA synthetase 2 antibody - C-terminal (ab72373)

Overview

  • Product name
    Anti-Alanyl-tRNA synthetase 2 antibody - C-terminal
    See all Alanyl-tRNA synthetase 2 primary antibodies
  • Description
    Rabbit polyclonal to Alanyl-tRNA synthetase 2 - C-terminal
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide from the C-terminal region of human Alanyl-tRNA synthetase 2 conjugated to KLH

  • Positive control
    • SK-BR-3 cell line lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Ammonium Sulphate Precipitation
  • Purification notes
    ab72373 is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab72373 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50 - 1/100. Detects a band of approximately 95 kDa (predicted molecular weight: 107 kDa).
ELISA 1/1000.
IHC-P 1/10 - 1/50.

Target

  • Relevance
    Alanyl-tRNA synthetase 2 (AARS2) is an enzyme that catalyzes the chemical reaction: ATP + L-alanine + tRNA(Ala) <---> AMP + diphosphate + L-alanyl-tRNA(Ala). This enzyme participates in alanine and aspartate metabolism and aminoacyl-tRNA biosynthesis.
  • Cellular localization
    Mitochondrion matrix
  • Database links
  • Alternative names
    • AARS 2 antibody
    • AARS2 antibody
    • AARSL antibody
    • Alanine tRNA ligase 2 mitochondrial antibody
    • Alanine tRNA ligase antibody
    • Alanyl tRNA synthetase 2 mitochondrial antibody
    • Alanyl tRNA synthetase like antibody
    • AlaRS antibody
    • bA444E17.1 antibody
    • COXPD8 antibody
    • Gm89 antibody
    • KIAA1270 antibody
    • LKENP antibody
    • MGC69820 antibody
    • MT ALARS antibody
    • MTALARS antibody
    • Probable alanyl tRNA synthetase mitochondrial antibody
    • RP11-444E17.1 antibody
    see all

Images

  • Anti-Alanyl-tRNA synthetase 2 antibody - C-terminal (ab72373) at 1/60 dilution + SK-BR-3 cell line lysate at 35 µg

    Predicted band size : 107 kDa
    Observed band size : 95 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.
  • ab72372 at 1/50 dilution staining Alanyl tRNA synthetase 2 C-terminal in human lung carcinoma tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). A peroxidase conjugated secondary antibody was used followed by DAB staining.

References

ab72373 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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