Overview

  • Product nameAnti-Alas1 antibody
    See all Alas1 primary antibodies
  • Description
    Mouse monoclonal to Alas1
  • Tested applicationsSuitable for: WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: MESVVRRCPF LSRVPQAFLQ KAGKSLLFYA QNCPKMMEVG AKPAPRALST AAVHYQQIKE TPPASEKDKT AKAKVQQTPD GSQQSPDGTQ LPSGHPLP, corresponding to amino acids 1-99 of Human Alas1

Properties

Applications

Our Abpromise guarantee covers the use of ab54758 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 71 kDa.
Flow Cyt Use 0.1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Target

  • PathwayPorphyrin metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from glycine: step 1/1.
  • Sequence similaritiesBelongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.
  • Cellular localizationMitochondrion matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • 5 aminolevulinate synthase antibody
    • 5 aminolevulinate synthase nonspecific mitochondrial antibody
    • 5 aminolevulinic acid synthase antibody
    • 5-aminolevulinate synthase antibody
    • 5-aminolevulinic acid synthase 1 antibody
    • Alas 1 antibody
    • ALAS 3 antibody
    • ALAS antibody
    • ALAS H antibody
    • ALAS HOUSEKEEPING TYPE antibody
    • ALAS N antibody
    • ALAS-H antibody
    • alaS1 antibody
    • ALAS3 antibody
    • ALASH antibody
    • Aminolevulinate delta synthase 1 antibody
    • Aminolevulinic acid synthase 1 antibody
    • Delta ALA synthetase antibody
    • Delta aminolevulinate synthase antibody
    • Delta-ALA synthase 1 antibody
    • Delta-aminolevulinate synthase 1 antibody
    • HEM1_HUMAN antibody
    • MIG 4 antibody
    • MIG4 antibody
    • Migration inducing protein 4 antibody
    • mitochondrial antibody
    • nonspecific antibody
    see all

Anti-Alas1 antibody images

  • Overlay histogram showing HepG2 cells stained with ab54758 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54758, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was an anti-mouse Alexa Fluor® 488 (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.



  • Predicted band size : 71 kDa
    Alas1 antibody (ab54758) at 1ug/lane + JAR cell lysate at 25ug/lane.

References for Anti-Alas1 antibody (ab54758)

This product has been referenced in:
  • Kubota Y  et al. Novel Mechanisms for Heme-dependent Degradation of ALAS1 Protein as a Component of Negative Feedback Regulation of Heme Biosynthesis. J Biol Chem N/A:N/A (2016). Read more (PubMed: 27496948) »

See 1 Publication for this product

Product Wall

Application Immunoprecipitation
Sample Human Cell lysate - whole cell (HepG2 cells)
Total protein in input 2000 µg
Treatment with/without 0.1 mM succinylaceton
Immuno-precipitation step Other - µMACS proteinG beads
Specification HepG2 cells
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Submitted Sep 20 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"