Recombinant
RabMAb

Anti-Alas1 antibody [EPR10247] - Mitochondrial Marker (ab154860)

Overview

  • Product name
    Anti-Alas1 antibody [EPR10247] - Mitochondrial Marker
    See all Alas1 primary antibodies
  • Description
    Rabbit monoclonal [EPR10247] to Alas1 - Mitochondrial Marker
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Alas1 aa 600 to the C-terminus. The exact sequence is proprietary.

  • Positive control
    • WB: HepG2, K562, JAR and Raji cell lysate. ICC/IF: HepG2 cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab154860 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 71 kDa.
ICC/IF 1/100 - 1/500.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Pathway
      Porphyrin metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from glycine: step 1/1.
    • Sequence similarities
      Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.
    • Cellular localization
      Mitochondrion matrix.
    • Information by UniProt
    • Database links
    • Alternative names
      • 5 aminolevulinate synthase antibody
      • 5 aminolevulinate synthase nonspecific mitochondrial antibody
      • 5 aminolevulinic acid synthase antibody
      • 5-aminolevulinate synthase antibody
      • 5-aminolevulinic acid synthase 1 antibody
      • Alas 1 antibody
      • ALAS 3 antibody
      • ALAS antibody
      • ALAS H antibody
      • ALAS HOUSEKEEPING TYPE antibody
      • ALAS N antibody
      • ALAS-H antibody
      • alaS1 antibody
      • ALAS3 antibody
      • ALASH antibody
      • Aminolevulinate delta synthase 1 antibody
      • Aminolevulinic acid synthase 1 antibody
      • Delta ALA synthetase antibody
      • Delta aminolevulinate synthase antibody
      • Delta-ALA synthase 1 antibody
      • Delta-aminolevulinate synthase 1 antibody
      • HEM1_HUMAN antibody
      • MIG 4 antibody
      • MIG4 antibody
      • Migration inducing protein 4 antibody
      • mitochondrial antibody
      • nonspecific antibody
      see all

    Images

    • Anti-Alas1 antibody [EPR10247] - Mitochondrial Marker (ab154860) at 1/10000 dilution (purified) + HepG2 whole cell lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 71 kDa
      Observed band size: 71 kDa



      Blocking and dilution buffer: 5% NFDM/TBST
    • Anti-Alas1 antibody [EPR10247] - Mitochondrial Marker (ab154860) at 1/10000 dilution (purified) + Raji whole cell lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 71 kDa
      Observed band size: 71 kDa



      Blocking and dilution buffer: 5% NFDM/TBST
    • Immunocytochemistry/Immunofluorescence analysis of HepG2 cells labelling Alas1 with purified ab154860 at a dilution of 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

      Control 1: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000).

      Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).

    • All lanes : Anti-Alas1 antibody [EPR10247] - Mitochondrial Marker (ab154860) at 1/1000 dilution (unpurified)

      Lane 1 : HepG2 cell lysate
      Lane 2 : K562 cell lysate
      Lane 3 : JAR cell lysate
      Lane 4 : Raji cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size: 71 kDa

    • Immunocytochemistry/Immunofluorescence analysis of HepG2 cells labeling Alas1 with unpurified ab154860 at a dilution of 1/250.

    References

    This product has been referenced in:
    • Kubota Y  et al. Novel Mechanisms for Heme-dependent Degradation of ALAS1 Protein as a Component of Negative Feedback Regulation of Heme Biosynthesis. J Biol Chem N/A:N/A (2016). Read more (PubMed: 27496948) »

    See 1 Publication for this product

    Customer reviews and Q&As

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HepG2 cells)
    Gel Running Conditions
    Reduced Denaturing (8% acrylamide gel)
    Loading amount
    10 µg
    Treatment
    with/without 0.1 mM succinylaceton
    Specification
    HepG2 cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Sep 21 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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