Anti-Alas1 antibody - Mitochondrial Marker (ab101681)

Overview

  • Product nameAnti-Alas1 antibody - Mitochondrial Marker
    See all Alas1 primary antibodies
  • Description
    Rabbit polyclonal to Alas1 - Mitochondrial Marker
  • Tested applicationsWB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Cow, Macaque Monkey
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human Alas1.

  • Positive control
    • This antibody gave a positive signal in HepG2 nuclear whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab101681 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).
ICC/IF Use a concentration of 5 µg/ml.

Target

  • PathwayPorphyrin metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from glycine: step 1/1.
  • Sequence similaritiesBelongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.
  • Cellular localizationMitochondrion matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • 5 aminolevulinate synthase antibody
    • 5 aminolevulinate synthase nonspecific mitochondrial antibody
    • 5 aminolevulinic acid synthase antibody
    • 5-aminolevulinate synthase antibody
    • 5-aminolevulinic acid synthase 1 antibody
    • Alas 1 antibody
    • ALAS 3 antibody
    • ALAS antibody
    • ALAS H antibody
    • ALAS HOUSEKEEPING TYPE antibody
    • ALAS N antibody
    • ALAS-H antibody
    • alaS1 antibody
    • ALAS3 antibody
    • ALASH antibody
    • Aminolevulinate delta synthase 1 antibody
    • Aminolevulinic acid synthase 1 antibody
    • Delta ALA synthetase antibody
    • Delta aminolevulinate synthase antibody
    • Delta-ALA synthase 1 antibody
    • Delta-aminolevulinate synthase 1 antibody
    • HEM1_HUMAN antibody
    • MIG 4 antibody
    • MIG4 antibody
    • Migration inducing protein 4 antibody
    • mitochondrial antibody
    • nonspecific antibody
    see all

Anti-Alas1 antibody - Mitochondrial Marker images

  • ICC/IF image of ab101681 stained T47D cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab101681, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab101681, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • Anti-Alas1 antibody - Mitochondrial Marker (ab101681) at 1 µg/ml + HepG2 (Human hepatocellular liver carcinoma cell line) Nuclear Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 70 kDa
    Observed band size : 70 kDa
    Additional bands at : 28 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes

References for Anti-Alas1 antibody - Mitochondrial Marker (ab101681)

ab101681 has not yet been referenced specifically in any publications.

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