Overview

  • Product nameAlbumin Mouse ELISA KitSee all Albumin kits ...
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Urine
  • Assay typeSandwich (quantitative)
  • Sensitivity
    = 1.5 ng/ml
  • Range
    1.56 ng/ml - 400 ng/ml
  • Recovery

    97.5 %

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam’s Albumin Mouse in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of mouse Albumin levels in urine and cell culture supernatants.

    An Albumin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Albumin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Albumin captured in plate.

  • Tested applicationsSandwich ELISAmore details
  • PlatformMicroplate

Properties

  • Cellular localizationSecreted.
  • Alternative names
    • ALB
    • albumin
    • Cell growth inhibiting protein 42
    • CSA
    • GIG20
    • GIG42
    • Growth inhibiting protein 20
    • PRO0883
    • PRO0903
    • PRO1708
    • PRO2044
    • PRO2619
    • PRO2675
    • UNQ696/PRO1341
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab108792 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Albumin Mouse ELISA Kit images

  • Representative Standard Curve Using ab108792.

Protocols

References for Albumin Mouse ELISA Kit (ab108792)

This product has been referenced in:
  • Kim SI  et al. TGF-ß-Activated Kinase 1 Is Crucial in Podocyte Differentiation and Glomerular Capillary Formation. J Am Soc Nephrol N/A:N/A (2014). Read more (PubMed: 24652804) »
  • Lakhe-Reddy S  et al. Mesangial cell avß8-integrin regulates glomerular capillary integrity and repair. Am J Physiol Renal Physiol 306:F1400-9 (2014). Read more (PubMed: 24740792) »

See all 2 Publications for this product

Product Wall


ab108792: We have never tested brain tissue supernatants so I do not know if it would work. I cannot recommend the dilution range.

ab119557: Again, we have not tested brain tissue with this kit but there is a publication where a FlowCyto...

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Thank you for your enquiry. I am sorry to hear the customer has some concerns regarding this kit, I hope I can be of help. For the standard,  as described in the protocol this needs to be reconstituted with the appropriate amount of diluent to gene...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"