Directly conjugated antibodies for immunofluorescence

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Overview

  • Product nameAlbumin Pig ELISA Kit
    See all Albumin kits
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 4.5%
  • Tests
    1 x 96 well plate
  • Sample type
    Serum, Plasma
  • Assay typeCompetitive
  • Sensitivity
    = 3 µg/ml
  • Range
    3.13 µg/ml - 100 µg/ml
  • Recovery

    98.5 %

  • Assay time
    3h 00m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Pig
  • Product overview

    Abcam’s Albumin Pig in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of pig Albumin levels in plasma and serum.

    An Albumin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Albumin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Albumin captured in plate.

  • Tested applicationsCompetitive ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionSerum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc.
  • Tissue specificityPlasma.
  • Involvement in diseaseFamilial dysalbuminemic hyperthyroxinemia
  • Sequence similaritiesBelongs to the ALB/AFP/VDB family.
    Contains 3 albumin domains.
  • Post-translational
    modifications    Kenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.
    Glycated in diabetic patients.
    Phosphorylation sites are present in the extracellular medium.
    Acetylated on Lys-223 by acetylsalicylic acid.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Alternative names
    • alb
    • ALBU_HUMAN
    • albumin
    • Cell growth inhibiting protein 42
    • CSA
    • GIG20
    • GIG42
    • Growth inhibiting protein 20
    • PRO0883
    • PRO0903
    • PRO1708
    • PRO2044
    • PRO2619
    • PRO2675
    • Serum albumin
    • UNQ696/PRO1341
    see all

Applications

Our Abpromise guarantee covers the use of ab108795 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA Use at an assay dependent concentration.

Albumin Pig ELISA Kit images

  • Typical Standard Curve
    Typical Standard Curve

    Representative Standard Curve using ab108795

Protocols

References for Albumin Pig ELISA Kit (ab108795)

ab108795 has not yet been referenced specifically in any publications.

Publishing research using ab108795 ? Please let us know so that we can cite the reference in this datasheet

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