Albumin Pig ELISA Kit (ab108795)
Overview
- Product nameAlbumin Pig ELISA KitSee all Albumin kits ...
- Detection methodColorimetric
- Precision
Intra-assay Sample n Mean SD CV% Overall 4.5% - Tests1 x 96 well plate
- Sample typeSerum, Plasma
- Assay typeCompetitive
- Sensitivity= 3 µg/ml
- Range3.13 µg/ml - 100 µg/ml
- Recovery
99 %
- Assay time3h 00m
- Assay durationMultiple steps standard assay
- Species reactivityReacts with: Pig
- Product overview
Abcam’s Albumin Pig in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of pig Albumin levels in plasma and serum.
An Albumin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Albumin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Albumin captured in plate.
- Tested applicationsCompetitive ELISA more details
- PlatformMicroplate
Properties
- Storage instructionsStore at +4°C. Please refer to protocols.
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Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate (SP Conjugate) 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 1 x 30ml 2X Biotinylated Pig Albumin Antibody (Lyophilized) 1 vial Albumin Microplate (12 x 8 well strips) 1 unit Albumin Standard 1 vial Chromogen Substrate 1 x 8ml Sealing Tapes 3 units Stop Solution 1 x 12ml -
Research Areas
- Cellular localizationSecreted.
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Alternative names
- ALB
- albumin
- Cell growth inhibiting protein 42
- CSA
- GIG20
- GIG42
- Growth inhibiting protein 20
- PRO0883
- PRO0903
- PRO1708
- PRO2044
- PRO2619
- PRO2675
- UNQ696/PRO1341
see all
Applications
Our Abpromise guarantee covers the use of ab108795 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Competitive ELISA | cELISA |
Albumin Pig ELISA Kit images
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Typical Standard CurveRepresentative Standard Curve using ab108795
Protocols
References for Albumin Pig ELISA Kit (ab108795)
ab108795 has not yet been referenced specifically in any publications.
