Anti-Alcohol Dehydrogenase antibody [EPR4439] (ab108203)

Overview

  • Product nameAnti-Alcohol Dehydrogenase antibody [EPR4439]
    See all Alcohol Dehydrogenase primary antibodies
  • Description
    Rabbit monoclonal [EPR4439] to Alcohol Dehydrogenase
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (Human)

  • Positive control
    • WB: lysate from HepG2 cells, Human fetal, mouse or rat liver; IHC-P: Human liver tissue; ICC/IF: HepG2 cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Alternative versions available:

    Anti-Alcohol Dehydrogenase antibody (HRP) [EPR4439] (ab202951)

Properties

Applications

Our Abpromise guarantee covers the use of ab108203 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500 - 1/1000.
WB 1/1000 - 1/10000. Predicted molecular weight: 40 kDa.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
  • Application notesIs unsuitable for Flow Cyt or IP.
  • Target

    Anti-Alcohol Dehydrogenase antibody [EPR4439] images

    • ab108203 staining Alcohol Dehydrogenase in the HepG2 cell line from Human liver by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde and blocked with 1% Serum for 60 minutes at 22°C. Samples were incubated with primary antibody (1/50) for 1 hour at 22°C. An Alexa Fluor®594-conjugated Donkey anti-rabbit polyclonal was used as the secondary antibody. Nucleus was stained with DAPI (blue).

      See Abreview

    • All lanes : Anti-Alcohol Dehydrogenase antibody [EPR4439] (ab108203) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : Mouse liver lysate
      Lane 3 : Human fetal liver lysate
      Lane 4 : Rat liver lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 40 kDa
    • ab108203 at 1/250 dilution staining Alcohol Dehydrogenase in paraffin-embedded Human liver tissue.
    • ab108203 at 1/500 dilution staining Alcohol Dehydrogenase in HepG2 cells by Immunofluorescence.

    References for Anti-Alcohol Dehydrogenase antibody [EPR4439] (ab108203)

    ab108203 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Sample Human Cell (HEPG-2)
    Specification HEPG-2
    Permeabilization No
    Fixative Formaldehyde
    Username

    Dr. Armen Petrosyan

    Verified customer

    Submitted Nov 04 2014

    Application Western blot
    Loading amount 25 µg
    Gel Running Conditions Reduced Denaturing (8% SDS-PAGE)
    Sample Human Cell lysate - whole cell (LNCaP)
    Specification LNCaP
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Dr. Armen Petrosyan

    Verified customer

    Submitted Oct 28 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"