This product is an IgG fraction antibody purified from monospecific antiserum by a multistep process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Dot: Use at an assay dependent dilution.
Immunoperoxidase Electron Microscopy: Use at an assay dependent dilution.
ELISA: 1/20000 - 1/100000.
WB: Use at an assay dependent dilution. Predicted molecular weight: 40 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Belongs to the zinc-containing alcohol dehydrogenase family.
References for Anti-Alcohol Dehydrogenase antibody (HRP) - Azide free (ab34575)
This product has been referenced in:
Anagnostis A & Papadopoulos AI Effects of a diet rich in sesame ( Sesamum indicum) pericarp on the expression of oestrogen receptor alpha and oestrogen receptor beta in rat prostate and uterus. Br J Nutr102:703-8 (2009).
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