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Abcam's Alcohol Dehydrogenase Detection Kit provides a convenient tool for sensitive detection of the Alcohol DH in a variety of samples. In the assay Alcohol DH will utilize isopropanol as a substrate leading to a proportional color development. The activity of ADH can be easily quantified colorimetrically (? = 450 nm). This assay detects ADH activity as low as 0.01 mU in samples.
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Alcohol dehydrogenase (Alcohol DH, ADH) (EC 188.8.131.52) is a group of seven dehydrogenase enzymes that occur in many organisms and facilitate the interconversion between alcohols and aldehydes or ketones with the reduction of NAD+ to NADH. In humans and many other animals, they serve to break down alcohols which could otherwise be toxic; in yeast and many bacteria, some alcohol dehydrogenases catalyze the opposite reaction as part of fermentation.
|ADH Assay Buffer||WM||1 x 25ml|
|ADH Positive Control (Lyophilized)||Green||1 vial|
|Developer (Lyophilized)||Red||1 vial|
|Isopropanol||Blue||1 x 1ml|
|NADH Standard (Lyophilized)||Yellow||1 vial|
Our Abpromise guarantee covers the use of ab102533 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|
Colorimetric standard curve: mean of duplicates (+/-SD) with background readings subtracted.
ab102533 has not yet been referenced specifically in any publications.