Anti-Aldehyde dehydrogenase 10 antibody (ab72774)

Overview

  • Product nameAnti-Aldehyde dehydrogenase 10 antibody
    See all Aldehyde dehydrogenase 10 primary antibodies
  • Description
    Mouse polyclonal to Aldehyde dehydrogenase 10
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    full-length human Aldehyde dehydrogenase 10. (NP_001026976.1).

  • Positive control
    • human liver lysate, Aldehyde dehydrogenase 10 transfected 293T cell line, HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab72774 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC/IF
  • Application notesICC/IF: Use at a concentration of 10 µg/ml. Note: ab72774 needs to be Protein A purified before use.
    WB: 1/500 - 1/1000. Predicted molecular weight: 55 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionCatalyzes the oxidation of long-chain aliphatic aldehydes to fatty acids. Active on a variety of saturated and unsaturated aliphatic aldehydes between 6 and 24 carbons in length.
    • Involvement in diseaseDefects in ALDH3A2 are the cause of Sjoegren-Larsson syndrome (SLS) [MIM:270200]. SLS is an autosomal recessive neurocutaneous disorder characterized by a combination of severe mental retardation, spastic di- or tetraplegia and congenital ichthyosis (increased keratinization). Ichthyosis is usually evident at birth, neurologic symptoms appear in the first or second year of life. Most patients have an IQ of less than 60. Additional clinical features include glistening white spots on the retina, seizures, short stature and speech defects.
    • Sequence similaritiesBelongs to the aldehyde dehydrogenase family.
    • Cellular localizationEndoplasmic reticulum membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Ahd 3 antibody
      • Ahd 3r antibody
      • Ahd3 antibody
      • Ahd3 r antibody
      • AL3A2_HUMAN antibody
      • Aldehyde dehydrogenase 10 antibody
      • Aldehyde dehydrogenase 3 antibody
      • Aldehyde dehydrogenase 3 family, member A2 antibody
      • Aldehyde dehydrogenase family 3 member A2 antibody
      • Aldehyde dehydrogenase family 3, subfamily A2 antibody
      • Aldehyde dehydrogenase, family 3, subfamily A, member 2 antibody
      • ALDH10 antibody
      • Aldh3 antibody
      • ALDH3A2 antibody
      • Aldh4 antibody
      • Aldh4 r antibody
      • Aldh4r antibody
      • DKFZp686E23276 antibody
      • FALDH antibody
      • Fatty aldehyde dehydrogenase antibody
      • FLJ20851 antibody
      • Microsomal aldehyde dehydrogenase antibody
      • msALDH antibody
      • SLS antibody
      see all

    Anti-Aldehyde dehydrogenase 10 antibody images

    • Anti-Aldehyde dehydrogenase 10 antibody (ab72774) at 1/500 dilution + human liver lysate at 50 µg

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 55 kDa
      Observed band size : 50 kDa (why is the actual band size different from the predicted?)
    • All lanes : Anti-Aldehyde dehydrogenase 10 antibody (ab72774) at 1/500 dilution

      Lane 1 : Aldehyde dehydrogenase 10 transfected 293T cell line
      Lane 2 : non transfected lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 55 kDa
      Observed band size : 51 kDa (why is the actual band size different from the predicted?)
    • Immunofluorescence analysis of Aldehyde dehydrogenase 10 expression in HeLa cells using 10 µg/ml purified ab72774

    References for Anti-Aldehyde dehydrogenase 10 antibody (ab72774)

    ab72774 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"