Aldehyde Quantification Assay Kit (Colorimetric) (ab112113)

Overview

  • Product nameAldehyde Quantification Assay Kit (Colorimetric)
    See all Aldehyde kits
  • Tests
    1 x 200 test
  • Sample type
    Saliva, Plasma, Cell culture extracts, Adherent cells, Suspension cells
  • Assay typeQuantitative
  • Species reactivity
    Reacts with: Human
  • Product overview

    ab112113 Aldehyde Quantification Assay Kit (Colorimetric) uses a proprietary dye that generates a chromogenic product upon reacting with an aldehyde. Most of the existing aldehyde test methods are based on separations either by the tedious and expensive HPLC-MS or GC-MS. ab112113 provides a sensitive, one-step colorimetric method to detect as little as 1 nanomole of aldehyde in a 100 µL assay volume (10 µM). The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and readily adapted to automation without a separation step. Its signal can be easily read with an absorbance microplate reader at 405 or 550 nm. This kit has been used for monitoring activities of oxidases that convert an amino group to an aldehyde group.

    Visit our FAQs page for tips and troubleshooting.

  • Notes

    ab112113 should be stored desiccated.

  • Tested applicationsFunctional Studiesmore details

Properties

    Applications

    Our Abpromise guarantee covers the use of ab112113 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Functional Studies Use at an assay dependent concentration.

    Aldehyde Quantification Assay Kit (Colorimetric) images

    • Aldehyde dose response was measured in a 96-well black plate with ab112113. As low as 10 µM (1 nanomol/well) of aldehyde can be detected with 30 minutes incubation (n=3). Standard curve was read at 405 nm.

    Protocols

    References for Aldehyde Quantification Assay Kit (Colorimetric) (ab112113)

    ab112113 has not yet been referenced specifically in any publications.

    Product Wall

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    1. The wavelength is mostly depending on the type of the aldehyde, and the incubation time. For example, Formaldehyde have only one peak 550nm, others have two peaks.

    2. Our standard is
    ...

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    The lab has not observed this before, and we have not received similar feedback from other customers. I would choose the values you obtained for the shorter assay incubation, rather than the overnight one. What you are seeing over time may in fact be t...

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    ab112113 can be used on plasma samples. The plasma samples don't need any special preparation so no spin columns are needed to deproteinise these samples.
    .

    The lab does not have experiences using this on fermentation broth. It should be OK theoretically, but you will need to subtract the background color of the broth from your reads.

    Hello and thank you for contacting us. The kit should work using the method you described, stopping the reaction, but we recommend using a basic solution (pH8) when doing so. Please let me know if you have any further questions.

    Thank you for your inquiry.
    I can confirm that the standard of ab112113 is Glyceradehyde (MW=90).
    I hope this information is helpful and that this kit is suitable for your experiments.

    Thank you for contacting us.
    Here are the answers to your questions:

    1) How much milligram of yellow indicator are there per vial? Answer: ˜ 24mg

    2) What is the exact chemistry of the kit ? Answer: The sensor contains an amine ...

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    Thank you for contacting Abcam regarding ab112113.


    Regarding the sample preparation for cell culture:


    You can either use freeze-thaw method (or homogenize for suspension cells) or simply use 0.5% Triton to lyse the cells. Read More

    Our lab justdid a quick experiment and foundthat Triton 100 up to 1% and NP-40 up to 0.5% has very little effect on the assay.

    I hope that this information is helpful.

    Please let me know if there is anything else I can help you with.

    Thank you for calling Abcam earlier today.

    I have talked to the lab and please find the answers to your questions below:

    1 - Will NP-40, or Triton affect this kit?---We haven't test it yet, will let you know as soon as we have the res...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"