For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
ALDH Activity Assay Kit (Colorimetric) (ab155893) is a simple, fast and reliable method to quantify the ALDH enzymatic activity. In this assay, acetaldehyde is oxidized by ALDH generating NADH which then reduces a colorless probe to a colored product with strong absorbance at 450 nm. The assay can detect < 0.5 mU of ALDH activity in a variety of samples.
The NAD-dependent Aldehyde Dehydrogenase (ALDH) plays a vital role in cellular detoxification. It oxidizes various aldehydes and generates the corresponding carboxyolic acid. ALDH have been found in every cellular compartment. Based on its structure and function, ALDH comprises 3 major classes in mammals: Class 1 and Class 3 (the tumor form) are located in the cytosol and include both constitutive and induced forms; Class 2 is located in the mitochondria and only exists as the constitutive form. In humans, the ALDH superfamily consists of 19 genes. The mutation of ALDH genes (loss of function) causes human diseases such as Type II hyperprolinemia, pyridoxine-dependent seizure and hyperammonemia. Recent studies show that increased ALDH activity leads to several types of malignancies, serves as a cancer stem cell marker and correlates with poor prognosis.
|Acetaldehyde||Purple||1 x 0.5ml|
|ALDH Assay Buffer||WM||1 x 25ml|
|ALDH Positive Control||Green||1 vial|
|ALDH Substrate Mix||Red||1 vial|
|NADH Standard||Yellow||1 vial|
Our Abpromise guarantee covers the use of ab155893 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"