Overview

  • Product name
    ALDH Activity Assay Kit (Colorimetric)
  • Sample type
    Cell culture supernatant, Serum, Plasma, Cell Lysate, Tissue Culture Media
  • Assay type
    Quantitative
  • Sensitivity
    < 0.5 mU/well
  • Species reactivity
    Reacts with: Mammal
  • Product overview

    ALDH Activity Assay Kit (Colorimetric) (ab155893) is a simple, fast and reliable method to quantify the ALDH enzymatic activity. In this assay, acetaldehyde is oxidized by ALDH generating NADH which then reduces a colorless probe to a colored product with strong absorbance at 450 nm. The assay can detect < 0.5 mU of ALDH activity in a variety of samples.

     

  • Notes

    The NAD-dependent Aldehyde Dehydrogenase (ALDH) plays a vital role in cellular detoxification. It oxidizes various aldehydes and generates the corresponding carboxyolic acid. ALDH have been found in every cellular compartment. Based on its structure and function, ALDH comprises 3 major classes in mammals: Class 1 and Class 3 (the tumor form) are located in the cytosol and include both constitutive and induced forms; Class 2 is located in the mitochondria and only exists as the constitutive form. In humans, the ALDH superfamily consists of 19 genes. The mutation of ALDH genes (loss of function) causes human diseases such as Type II hyperprolinemia, pyridoxine-dependent seizure and hyperammonemia. Recent studies show that increased ALDH activity leads to several types of malignancies, serves as a cancer stem cell marker and correlates with poor prognosis.

  • Tested applications
    Suitable for: Functional Studiesmore details

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components Identifier 100 tests
    Acetaldehyde Purple 1 x 0.5ml
    ALDH Assay Buffer WM 1 x 25ml
    ALDH Positive Control Green 1 vial
    ALDH Substrate Mix Red 1 vial
    NADH Standard Yellow 1 vial
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab155893 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

Images

  • Standard curve (60 minutes incubation) showing mean of duplicates (+/- SD) with background reads subtracted.

  • ALDH activity measured in human biological fluids (T1=5 min; T2=60 min), showing activity (mU) per mL of tested sample, background signal subtracted.

Protocols

References

This product has been referenced in:
  • Norona LM  et al. Editor's Highlight: Modeling Compound-Induced Fibrogenesis In Vitro Using Three-Dimensional Bioprinted Human Liver Tissues. Toxicol Sci 154:354-367 (2016). Functional Studies . Read more (PubMed: 27605418) »

See 1 Publication for this product

Customer reviews and Q&As

The ALDH Activity Assay Kit (Colorimetric) (ab155893) has not been tested yet in cancer cell lines such as MDA-MB-231 and MCF-7.
Unfortunately, we do not currently have more information about this product. We will update the on-line datasheet for ...

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The contents of the substrate mix is proprietary information and cannot be disclosed.
The positive control is an ALDH isolated from Baker’s yeast.

We would recommend using our assay buffer for the lysis, as RIPA may not contain all the components required for the reaction to occur. The samples should be homogenized in ice cold assay buffer for 10 minutes on ice (page 8 of the online protocol). L...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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