Overview

  • Product name
    Anti-ALDH1A1 antibody [5A11]
    See all ALDH1A1 primary antibodies
  • Description
    Mouse monoclonal [5A11] to ALDH1A1
  • Tested applications
    Suitable for: WB, IHC-P, ELISA, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Purified recombinant fragment of Human ALDH1A1 expressed in E. coli.

  • Positive control
    • WB: Raji, Jurkat, THP1 and K562 cell lysates IHC-P: Human cerebellum and lung cancer tissues
  • General notes

    This product was changed from ascites to supernatant. Lot no’s high than GR120645-17 are from Tissue Culture Supernatant

Properties

Applications

Our Abpromise guarantee covers the use of ab105920 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200 - 1/1000. Predicted molecular weight: 55 kDa.
IHC-P 1/200 - 1/1000.
ELISA 1/10000.
Flow Cyt 1/100.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Binds free retinal and cellular retinol-binding protein-bound retinal. Can convert/oxidize retinaldehyde to retinoic acid.
  • Pathway
    Cofactor metabolism; retinol metabolism.
  • Sequence similarities
    Belongs to the aldehyde dehydrogenase family.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Acetaldehyde dehydrogenase 1 antibody
    • AHD2 antibody
    • AL1A1_HUMAN antibody
    • ALDC antibody
    • Aldehyde dehydrogenase 1 family member A1 antibody
    • Aldehyde dehydrogenase 1 soluble antibody
    • Aldehyde dehydrogenase 1A1 antibody
    • Aldehyde dehydrogenase antibody
    • Aldehyde dehydrogenase cytosolic antibody
    • Aldehyde dehydrogenase family 1 member A1 antibody
    • Aldehyde dehydrogenase liver cytosolic antibody
    • ALDH 1 antibody
    • ALDH 1A1 antibody
    • ALDH class 1 antibody
    • ALDH, liver cytosolic antibody
    • ALDH-E1 antibody
    • ALDH1 A1 antibody
    • ALDH1 antibody
    • ALDH11 antibody
    • ALDH1A1 antibody
    • ALHDII antibody
    • cytosolic antibody
    • epididymis luminal protein 12 antibody
    • epididymis luminal protein 9 antibody
    • epididymis secretory sperm binding protein Li 53e antibody
    • HEL-S-53e antibody
    • MGC2318 antibody
    • PUMB1 antibody
    • RALDH 1 antibody
    • RalDH1 antibody
    • Retinal dehydrogenase 1 antibody
    see all

Anti-ALDH1A1 antibody [5A11] images

  • Overlay histogram showing HepG2 cells stained with ab105920 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab105920, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  • All lanes : Anti-ALDH1A1 antibody [5A11] (ab105920) at 1/200 dilution

    Lane 1 : Raji cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : THP1 cell lysate
    Lane 4 : K562 cell lysate


    Predicted band size : 55 kDa
  • ab105920, at a 1/200 dilution, staining ALDH1A1 in paraffin embedded Human cerebellum tissue by Immunohistochemistry.
  • ab105920, at a 1/200 dilution, staining ALDH1A1 in paraffin embedded Human lung cancer tissue by Immunohistochemistry.

References for Anti-ALDH1A1 antibody [5A11] (ab105920)

This product has been referenced in:
  • Jia D  et al. Cardamonin reduces chemotherapy-enriched breast cancer stem-like cells in vitro and in vivo. Oncotarget 7:771-85 (2016). WB . Read more (PubMed: 26506421) »
  • Martinez-Cruzado L  et al. Aldh1 Expression and Activity Increase During Tumor Evolution in Sarcoma Cancer Stem Cell Populations. Sci Rep 6:27878 (2016). WB, IF . Read more (PubMed: 27292183) »

See all 2 Publications for this product

Product Wall

Thank you for contacting us. ab3380; The ABCG2 target is expressed in tonsils, spleen, gall bladder and lungs etc so the sections of these tissues will be good positive control. You can find the complete expression information on Human Protein Atlas w...

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Thank you for contacting us. Unfortunately we do not have an antibody that suits your needs. All of the ALDH1A1 antibodies have either been tested in mouse or have sufficient homology (greater than 80%) with the immunogen so that they are likely to rea...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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