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Synthetic peptide corresponding to residues near the C-terminus of human Aldh1a1
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Alternative versions available:
Anti-ALDH1A1 antibody (Alexa Fluor® 488) [EP1933Y] (ab195254)
Anti-ALDH1A1 antibody (Alexa Fluor® 594) [EP1933Y] (ab206884)
Anti-ALDH1A1 antibody (Alexa Fluor® 647) [EP1933Y] (ab195255)
Anti-ALDH1A1 antibody (HRP) [EP1933Y] (ab195517)
Anti-ALDH1A1 antibody (Phycoerythrin) [EP1933Y] (ab209437)
Our Abpromise guarantee covers the use of ab52492 in the following tested applications.
|ICC/IF||1/100 - 1/250.|
|WB||1/500 - 1/2000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|IHC-P||Use at an assay dependent concentration.|
Immunocytochemistry/ Immunofluorescence analysis of HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling ALDH1A1 with ab52492 at 1/500 dilution (4 μg/ml). Cells were fixed in 100% methanol. ab150077, an AlexaFluor®488 Goat anti-Rabbit secondary antibody was used at 1/1000 dilution (2 μg/ml). Ab195889, Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain at 1/200 dilution (2.5 μg/ml). DAPI was used as nuclear counterstain. Confocal image showing cytoplasmic staining on HepG2 cell line. No staining on MCF-7 cell line.
Overlay histogram showing HepG2 cells stained with ab52492 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52492, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.