Anti-ALDH1A1 antibody - Neuronal Marker (ab24343)

Overview

  • Product nameAnti-ALDH1A1 antibody - Neuronal Marker
    See all ALDH1A1 primary antibodies
  • Description
    Rabbit polyclonal to ALDH1A1 - Neuronal Marker
  • SpecificityMay cross-react with mouse ALDH1A2 and ALDH1A3
  • Tested applicationsSuitable for: IHC-P, ICC/IF, WB, IHC-FoFr, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Full length mouse ALDH1A1 protein (GenBank NM_013467) expressed as a fusion protein (Glutathione-S-Transferase) in E. coli.

  • Positive control
    • ALDH1A1 protein is a known mesencephalic dopaminergic marker.

Properties

Applications

Our Abpromise guarantee covers the use of ab24343 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
ICC/IF 1/200.
WB Use at an assay dependent concentration.
IHC-FoFr 1/100.
IHC-Fr 1/100.

Target

  • FunctionBinds free retinal and cellular retinol-binding protein-bound retinal. Can convert/oxidize retinaldehyde to retinoic acid.
  • PathwayCofactor metabolism; retinol metabolism.
  • Sequence similaritiesBelongs to the aldehyde dehydrogenase family.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Acetaldehyde dehydrogenase 1 antibody
    • AHD2 antibody
    • AL1A1_HUMAN antibody
    • ALDC antibody
    • Aldehyde dehydrogenase 1 family member A1 antibody
    • Aldehyde dehydrogenase 1 soluble antibody
    • Aldehyde dehydrogenase 1A1 antibody
    • Aldehyde dehydrogenase antibody
    • Aldehyde dehydrogenase cytosolic antibody
    • Aldehyde dehydrogenase family 1 member A1 antibody
    • Aldehyde dehydrogenase liver cytosolic antibody
    • ALDH 1 antibody
    • ALDH 1A1 antibody
    • ALDH class 1 antibody
    • ALDH, liver cytosolic antibody
    • ALDH-E1 antibody
    • ALDH1 A1 antibody
    • ALDH1 antibody
    • ALDH11 antibody
    • ALDH1A1 antibody
    • ALHDII antibody
    • cytosolic antibody
    • epididymis luminal protein 12 antibody
    • epididymis luminal protein 9 antibody
    • epididymis secretory sperm binding protein Li 53e antibody
    • HEL-S-53e antibody
    • MGC2318 antibody
    • PUMB1 antibody
    • RALDH 1 antibody
    • RalDH1 antibody
    • Retinal dehydrogenase 1 antibody
    see all

Anti-ALDH1A1 antibody - Neuronal Marker images

  • ALDH1A1 antibody (ab24343) staining [A] tyrosine hydroxylase (TH; green) and [B] ALDH1A1 (red) -containing neurons in adult mouse brain sections by immunohistochemistry. [C] Overlap of TH and ALDH1A1-containing neurons. Pictures were taken with 20x objective. Sections were fixed in 4% formaldehyde for 30 min, rinsed with PBS and then incubated with blocking buffer (PBS, 10 % normal donkey serum; NDS) for 10 min. Sections were then incubated overnight at 4 degrees C with primary antibodies diluted in PBS containing 2% NDS. After additional rinsing in PBS, the coverslips were incubated in fluorescent-labeled secondary antibodies (Cy2-, Rhodamine Red-X-, or Cy5-labeled donkey IgG) in PBS with 2% NDS for 30 min at RT.

    ALDH1A1 antibody (ab24343) staining [A] tyrosine hydroxylase (TH; green) and [B] ALDH1A1 (red) -containing neurons in adult mouse brain sections by immunohistochemistry. [C] Overlap of TH and ALDH1A1-containing neurons. Pictures were taken with 20x objective. Sections were fixed in 4% formaldehyde for 30 min, rinsed with PBS and then incubated with blocking buffer (PBS, 10 % normal donkey serum; NDS) for 10 min. Sections were then incubated overnight at 4 degrees C with primary antibodies diluted in PBS containing 2% NDS. After additional rinsing in PBS, the coverslips were incubated in fluorescent-labeled secondary antibodies (Cy2-, Rhodamine Red-X-, or Cy5-labeled donkey IgG) in PBS with 2% NDS for 30 min at RT.

  • ab24343 staining ALDH1A1 - Neuronal Marker (green) in Mouse pancreatic neoplasia tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone/methanol (1:1) and blocked with 10% serum for 1 hour at room temperature. Samples were incubated with primary antibody (1/100 in PBS) for 4 hours. An Alexa Fluor®488-conjugated Donkey anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody. Nuclei were stained by DAPI (blue).

    See Abreview

References for Anti-ALDH1A1 antibody - Neuronal Marker (ab24343)

This product has been referenced in:
  • Rolland AS  et al. Expression mediated by three partial sequences of the human tyrosine hydroxylase promoter in vivo. Mol Ther Methods Clin Dev 3:16062 (2016). Read more (PubMed: 27689101) »
  • Bifsha P  et al. Rgs6 is required for adult maintenance of dopaminergic neurons in the ventral substantia nigra. PLoS Genet 10:e1004863 (2014). IHC-P ; Mouse . Read more (PubMed: 25501001) »

See all 11 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer
Sample Mouse Tissue sections (Pancreatic cancer)
Specification Pancreatic cancer
Permeabilization Yes - PBS+Triton 0.05%
Fixative Formaldehyde
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Submitted Dec 25 2014

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this h...

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Thank you for your new contact.

My colleague is out of office today. Checking the stock availability, it looks that we have only one batch of the ALDH1A1 antibody that was sent to you. We can offer you a new aliquot if you wish or a credit no...

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Thanks for the information.

We had orders from XXXXXXXXXXX in year 2008, the lot we sent was 369562. This lot is currently out of stock, so I am sorry we will not be able to send this lot. My suggestion would be to try a new vial of same lot h...

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Thank you very much for your email.

We generally recommend our customers trying 5-10 micron sections. I can recommend 8 micron thick sections than 16; the results will be much nicer. This could be reason why the antibody is not giving high int...

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Thank you very much for your email. I am sorry to hear to hear that you are having problems with this antibody.

We have not received any other complaint for this batch so the problem might be with the vial or with the protocol. In order to und...

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Thank you very much for your email. I am sorry to hear to hear that you are having problems with this antibody.

We have not received any other complaint for this batch so the problem might be with the vial or with the protocol. In order to un...

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Unfortunately the immunogen for ALDH1A1 is not available for sale.



At this timeonepossible way toobtain the protein is likely toa obtain theplasmid expressing mouse ALDH1A1 in E. coli or some other vector, and then isolate the prot...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (putative pancreatic cancer stem cell fraction)
Specification putative pancreatic cancer stem cell fraction
Fixative Formaldehyde
Permeabilization Yes - PBST
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
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Verified customer

Submitted Oct 01 2010

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (pancreas)
Specification pancreas
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 16°C
Username

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Verified customer

Submitted Sep 28 2010

1-10 of 16 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"