Overview

  • Product name
  • Description
    Rabbit polyclonal to ALDH1A2
  • Tested applications
    Suitable for: WB, IHC-Fr, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Cow, Dog, Human, Monkey
    Predicted to work with: Rat, Chicken
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Human ALDH1A2.

    (Peptide available as ab101078.)

  • Positive control
    • This antibody gave a positive signal in Human brain and liver tissue lysates and FFPE human testis tissue sections.

Properties

Applications

Our Abpromise guarantee covers the use of ab75674 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
IHC-Fr Use at an assay dependent concentration.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function
    Recognizes as substrates free retinal and cellular retinol-binding protein-bound retinal. Does metabolize octanal and decanal but does not metabolize citral, benzaldehyde, acetaldehyde and propanal efficiently.
  • Pathway
    Cofactor metabolism; retinol metabolism.
  • Sequence similarities
    Belongs to the aldehyde dehydrogenase family.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • AL1A2_HUMAN antibody
    • Aldehyde dehydrogenase family 1 member A2 antibody
    • ALDH1A2 aldehyde dehydrogenase 1 family, member A2 antibody
    • ALDH1A2 antibody
    • Aldh1a7 antibody
    • AV116159 antibody
    • MGC26444 antibody
    • RALDH 2 antibody
    • RALDH(II) antibody
    • Raldh1 antibody
    • RalDH2 antibody
    • RALDH2 T antibody
    • Retinal dehydrogenase 2 antibody
    • Retinaldehyde dehydrogenase 2 antibody
    • Retinaldehyde specific dehydrogenase type 2 antibody
    • Retinaldehyde-specific dehydrogenase type 2 antibody
    see all

Images

  • All lanes : Anti-ALDH1A2 antibody (ab75674) at 1 µg/ml

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : Human brain tissue lysate - total protein (ab29466)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 56 kDa
    Observed band size : 56 kDa
    Additional bands at : 17 kDa,46 kDa,73 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes
  • IHC image of ab75674 staining in human testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab75674, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  • ab75674 staining ALDH1A2 on E9.5 mouse embryo sections by immunohistochemistry. The tissue was paraformaldehyde fixed and permeabilized in PBST prior to blocking in NOVOCASTRA protein block for 30 minutes at 25°C. The primary antibody was diluted 1/500 and incubated with the sample for 48 hour at 4°C. An Alexa Fluor 555 conjugated donkey anti-rabbit antibody, diluted 1/1000, was used as the secondary. Nuclear staining shown with DAPI.

    See Abreview

References

This product has been referenced in:
  • Cano E  et al. Extracardiac septum transversum/proepicardial endothelial cells pattern embryonic coronary arterio-venous connections. Proc Natl Acad Sci U S A 113:656-61 (2016). IHC . Read more (PubMed: 26739565) »
  • Sugimoto R  et al. Retinoic acid metabolism links the periodical differentiation of germ cells with the cycle of Sertoli cells in mouse seminiferous epithelium. Mech Dev 128:610-24 (0). Read more (PubMed: 22200512) »

See all 2 Publications for this product

Customer reviews and Q&As

Application
Western blot
Loading amount
100 µg
Gel Running Conditions
Reduced Denaturing (12)
Sample
Cow Cell lysate - whole cell (MDBK cells)
Specification
MDBK cells
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
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Submitted Oct 18 2013

Application
Western blot
Loading amount
100 µg
Gel Running Conditions
Reduced Denaturing (12)
Sample
Dog Cell lysate - whole cell (MDCK cells)
Specification
MDCK cells
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
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Submitted Oct 18 2013

Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (12)
Sample
Human Cell lysate - whole cell (HEK293F cells)
Specification
HEK293F cells
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
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Submitted Oct 18 2013

Application
Western blot
Loading amount
100 µg
Gel Running Conditions
Reduced Denaturing (12)
Sample
Mouse Tissue lysate - whole (Mouse kidney tissue lysate)
Specification
Mouse kidney tissue lysate
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
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Submitted Oct 18 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Dog Cell lysate - whole cell (MDCK cells)
Loading amount
100 µg
Specification
MDCK cells
Gel Running Conditions
Reduced Denaturing (Tris-Glycine -10%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Feb 20 2013

Application
Western blot
Sample
African Green Monkey Cell lysate - whole cell (COS cells)
Loading amount
100 µg
Specification
COS cells
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

Submitted Feb 05 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (E9.5 mouse embryo sections)
Specification
E9.5 mouse embryo sections
Fixative
Paraformaldehyde
Permeabilization
Yes - PBST (PBS/0.5%v/v TritonX100 (Sigma)
Blocking step
NOVOCASTRA protein block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
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Verified customer

Submitted Oct 24 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (e7.5)
Loading amount
2 µg
Specification
e7.5
Gel Running Conditions
Reduced Denaturing (9% gel)
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 4°C
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Verified customer

Submitted Aug 31 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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